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  • PCR 1: A Practical Approach

    PCR 1 by McPherson, M. J.; Quirke, P.; Taylor, G. R.;

    A Practical Approach

    Sorozatcím: Practical Approach Series; 77;

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    46 819 Ft

    Beszerezhetőség

    A kiadónál véglegesen elfogyott, nem rendelhető. Érdemes újra keresni a címmel, hátha van újabb kiadás.

    Why don't you give exact delivery time?

    A beszerzés időigényét az eddigi tapasztalatokra alapozva adjuk meg. Azért becsült, mert a terméket külföldről hozzuk be, így a kiadó kiszolgálásának pillanatnyi gyorsaságától is függ. A megadottnál gyorsabb és lassabb szállítás is elképzelhető, de mindent megteszünk, hogy Ön a lehető leghamarabb jusson hozzá a termékhez.

    A termék adatai:

    • Kiadó IRL Press
    • Megjelenés dátuma 1991. szeptember 5.

    • ISBN 9780199631964
    • Kötéstípus Puhakötés
    • Terjedelem274 oldal
    • Méret 233x147x15 mm
    • Súly 478 g
    • Nyelv angol
    • Illusztrációk numerous halftones, line drawings, tables
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    Rövid leírás:

    Another extremely important title. The value of Polymerase Chain Reaction (PCR) cannot be over-stated. It has become one of the most widely used techniques in biomedical research, enabling the fast, inexpensive production of large quantities of DNA from minute amounts of starting material. Although the basic procedure is very simple, many variations of it have been developed for different applications, for use in such diverse areas as forensic and diagnostic
    medicine, genetic engineering, and the food industry. PCR: A Practical Approach provides a general introduction to PCR for researchers new to the area, followed by chapters detailing specific applications of the technique. The emphasis throughout is on the practical applications of the technique and detailed
    experimental protocols are included at all stages. A starting point for those new to PCR and will also serve as a useful reference for more experienced researchers in all areas of biological science.

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    Hosszú leírás:

    The Polymerase Chain Reaction (PCR) has become one of the most widely used techniques in biomedical research, enabling the fast, inexpensive production of large quantities of DNA from minute amounts of starting material. Although the basic procedure is very simple, many variations of it have been developed for different applications, for use in such diverse areas as forensic and diagnostic medicine, genetic engineering, and the food industry.

    PCR: A Practical Approach provides a general introduction to PCR for researchers new to the area, followed by chapters detailing specific applications of the technique. It covers gene analysis at the level of restriction enzyme polymorphism, point mutation and sequence analysis. Methods for using different source materials - cloned DNA, genomic DNA, RNA, nucleic acid from archive material and PCR products themselves - are given.

    The emphasis throughout is on the practical applications of the technique and detailed experimental protocols are included at all stages, backed up by advice on avoiding potential pitfalls. This volume provides a starting point for those new to PCR and will also serve as a useful reference for more experienced researchers in all areas of biological science.

    'This volume provides a starting point for those new to PCR and will also serve as a useful reference for more experienced researchers in all areas of biological science.'
    Biotech Products International, Sept/Oct. 1991 Vol3-No9/10

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    Tartalomjegyzék:

    Graham R. Taylor: Polymerase chain reaction: basic principles and automation; Adrian Ivinson, & Graham R. Taylor: Polymerase chain reaction in genetic diagnosis; David P. Jackson, Julie D. Hayden, & Philip Quirke: Extraction of nucleic acid from fresh and archival material; Roland G. Roberts, A. Jane Montandon, Peter M. Green, & David R. Bentley: Analysis of genomic sequence variation using amplification and mismatch detection; Belinda J.F. Rossiter,
    Markus Grompe, & C. Thomas Caskey: Detection of deletions and point mutations; Michael Litt: PCR of TG microsatellites; Susan A. Ledbetter, & David L. Nelson: Genome amplification using primers directed to interspersed repetitive sequences (IRS-PRC); Daniel H. Johnson: PCR amplification of microdissected DNA;
    Jonathon Silver: Inverse polymerase chain reaction; Sarah Jane Gurr, & Michael J. McPherson: PCR-directed cDNA libraries; Michael J. McPherson, Kerrie M. Jones, & Sarah Jane Gurr: PCR with highly redundant primers; Tim Clackson, Detlef Gussow, & Peter T. Jones: General application of PCR to gene cloning and manipulation; Margaret J. Dallman, & Andrew C.G. Porter: Semi-quantitative PCR for the analysis of gene expression; Kirstin A. Eckert, & Thomas A. Kunkel: The fidelity of
    DNA polymerases used in the PCR; Appendix, Index

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