An Introduction to Single Molecule Biophysics by Lyubchenko, Yuri L.;

Rövid leírás:

This introductory book broadly covers the foundations, principles, and methodologies of single molecule fluorescence and force probe techniques as applied to the most important and timely biological problems, providing a first introduction to key concepts and highlights the advantages and pitfalls of each of these exciting emerging methods.

Hosszú leírás:

This book gives an accessible, detailed overview on techniques of single molecule biophysics (SMB), showing how they are applied to numerous biological problems associated with understanding the molecular mechanisms of DNA replication, transcription, and translation, as well as functioning of molecular machines. It covers major single molecule imaging and probing techniques, highlighting key strengths and limitations of each method using recent examples. The chapters begin with a discussion of single molecule fluorescence techniques followed by an overview of the atomic force microscope and its use for direct time-lapse visualization of dynamics of molecular complexes at the nanoscale, as well as applications in measurements of interactions between molecules and mechanical properties of isolated molecules and their complexes. The next chapters address magnetic tweezers and optical tweezers, including instrumentation, fundamentals of operation, and applications. A final chapter turns to nanopore transport and nanopore-based DNA sequencing technology that will play a major role in next-generation genomics and healthcare applications.

Tartalomjegyzék:

1. Single Molecule Fluorescence

2. Atomic Force Microscope for Topographic Studies

3. Atomic Force Microscope Force Spectroscopy

4. Magnetic Tweezers

5. Optical Tweezers

6. Nanofluidic Transport and Sensing in Biological and Artificial Nanopores