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  • Exosomes and Microvesicles: Methods and Protocols

    Exosomes and Microvesicles by Hill, Andrew F;

    Methods and Protocols

    Series: Methods in Molecular Biology; 1545;

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      • Publisher's listprice EUR 106.99
      • The price is estimated because at the time of ordering we do not know what conversion rates will apply to HUF / product currency when the book arrives. In case HUF is weaker, the price increases slightly, in case HUF is stronger, the price goes lower slightly.

        44 374 Ft (42 261 Ft + 5% VAT)
      • Discount 20% (cc. 8 875 Ft off)
      • Discounted price 35 499 Ft (33 809 Ft + 5% VAT)

    44 374 Ft

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    Product details:

    • Edition number Softcover reprint of the original 1st ed. 2017
    • Publisher Springer New York
    • Date of Publication 7 July 2018
    • Number of Volumes 1 pieces, Previously published in hardcover

    • ISBN 9781493982844
    • Binding Paperback
    • See also 9781493967261
    • No. of pages228 pages
    • Size 254x178 mm
    • Weight 463 g
    • Language English
    • Illustrations XII, 228 p. 39 illus., 21 illus. in color. Illustrations, black & white
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    Long description:

    This volume covers methods for the analysis of extracellular vesicles (EV) that can be applied to isolated EVs from a wide variety of sources. This includes the use of electron microscopy, tunable resistance pulse sensing, and nanoparticle tracking analysis. The chapters in this book discuss EV cargoes containing proteins and genomic materials using a number of different approaches, and isolating EVs from platelets and neuronal cells and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

    Practical and comprehensive, Exosomes and Microvesicles: Methods and Protocols is a valuable resource containing methodologies for anyone interested in researching EVs.

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    Table of Contents:

    Methods to Analyze EVs.- Tunable Resistive Pulse Sensing for the Characterization of Extracellular Vesicles.- Immuno-Characterization of Exosomes using Nanoparticle Tracking Analysis.- Imaging and Quantification of Extracellular Vesicles by Transmission Electron Microscopy.- Quantitative Analysis of Exosomal miRNA via qPCR and Digital PCR.- Small RNA Library Construction for Exosomal RNA from Biological Samples for the Ion Torrent PGMTM System.- A Protocol for Isolation and Proteomic Characterization of Distinct Extracellular Vesicle Subtypes by Sequential Centrifugal Ultrafiltration.- Multiplexed Phenotyping of Extracellular Vesicles using Protein Microarray (EV Array).- Purification and Analysis of Exosomes Released by Mature Cortical Neurons following Synaptic Activation.- A Method for Isolation of Extracellular Vesicles and Characterization of Exosomes from Brain Extracellular Space.- Isolation of Exosomes and Microvesicles from Cell Culture Systems to Study Prion Transmission.- Isolation of Platelet-Derived Extracellular Vesicles.- Bioinformatics Tools for Extracellular Vesicles Research.- Preparation and Isolation of siRNA-Loaded Extracellular Vesicles.- Interaction of Extracellular Vesicles with Endothelial Cells under Physiological Flow Conditions.

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