Immunology: Overview and Laboratory Manual by Sam-Yellowe, Tobili Y.;

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A termék adatai:

Kiadás sorszáma 2
Kiadó Springer International Publishing
Megjelenés dátuma 2026. július 14.

ISBN 9783032199973
Kötéstípus Keménykötés
Terjedelem589 oldal
Méret 235x155 mm
Nyelv angol
Illusztrációk XX, 589 p. 80 illus., 42 illus. in color.

Kategóriák

A biológia általános kérdései Sejttan Az orvostudomány általános kérdései Immunológia, allergológia A biológia általános kérdései (karitatív célú kampány) Sejttan (karitatív célú kampány) Az orvostudomány általános kérdései (karitatív célú kampány) Immunológia, allergológia (karitatív célú kampány)

Hosszú leírás:

"

A second edition, two-in-one text to be utilized in providing students with an overview of immunology, as well as a lab manual complete with current standard exercises.

Section I of this book provides an overview of the immune system and immunity, and includes review questions, problem sets, case studies, inquiry-based questions, and more to provide students with a strong foundation in the field. New chapters on trained immunity, oral immunity, mucosal immunity, chimeric antigen receptor (CAR) T cells, and immune checkpoint inhibitors have been added to the second edition. Section II consists of thirty-seven lab exercises focused on key concepts in immunology, such as antibody production, cell separation, cell function, immunoassays, Th1/Th2 cytokine detection, chromatin immunoprecipitation (ChIP), nitric oxide detection, cell and tissue culture methods, and cell and molecular biology techniques. Appendices include safety information, suggested links and readings, and standard discipline processes, protocols, and instructions.

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Több

Tartalomjegyzék:

Part I: Overview of Immunity.- Ch 1: Immunity.- Ch 2: Components of immunity.- Ch 3: Hematopoiesis.- Ch 4: The Immune system: pathogen sensing and detection.- Ch 5: Lymphoid organs.- Ch 6: Innate and Adaptive immunity.- Ch 7: Leukocyte homing, migration and recirculation.- Ch 8: Apoptosis.- Ch 9: Antigens.- Ch 10: B cell development, activation and immunoglobulin structure.- Ch 11: Immunoglobulin and T cell receptor gene rearrangements.- Ch 12: Major histocompatibility complex proteins.- Ch 13: T cell development and T cell receptor structure.- Ch 14: Cytokines.- Ch 15: Lymphocyte signals.- Ch 16: Complement fixation.- Ch 17: Cell mediated immunity.- Ch 18: Hypersensitivities.- Ch 19: Parasite Immunity.- Ch 20: Vaccines.- Ch 21: Transplantation.- Ch 22: Immune regulation and autoimmunity.- Ch 23: Immunodeficiencies.- Ch 24: Cancer.- Ch 25: Practice questions.- Ch 26: Study guide for exam. Part II. Laboratory Manual.- 27. Immunology laboratory policies.- 28. Instructions for notebook entries.- 29. Instructions for writing lab reports.- 30. Lab report topics.- 31. Exercise 1: Using a compound microscope.- 32. Exercise 2: Pipetting and dilution technique, identification of mouse lymphoid organs.- 33. Exercise 3: Blood Cell Preparation, Leukocyte differentiation and ABO Blood Typing.- 34. Exercise 4: Single Suspension of Mouse Spleen cells, Cell Viability Assays, and Identification of specific cells using surface antigens.- 35. Exercise 5: Isolation of mouse peritoneal macrophages: In vitro phagocytosis of bacterial cells.- 36. Exercise 6: Clearance of bacteria from mouse blood by the RES.- 37. Exercise 7: Hemolytic Plaque assay (The Jeme Plaque Assay).- 38. EXERCISE 8: Rabbit and Mouse Immunizations- Preparation of Polyclonal antibodies and screening assays.- 39. EXERCISE 9: Determination of Antibody Titer: Screening Techniques.- 40. EXERCISE 10: Immunodiffusion and Immunoelectrophoresis.- 41. EXERCISE 11: Protein Affinity Purification of Rabbit IgG.- 42. EXERCISE 12: Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE).- 43. EXERCISE 13: Immunoblotting (Western Blotting).- 44. Exercise 14: Protein Assay.- 45. Exercise 15: Enzyme-linked Immunosorbent Assay (ELISA).- 46. EXERCISE 16: Enzyme-Linked Immunospot Assay (ELISPOT).- 47. EXERCISE 17: Immunofluorescence assay (IFA).- 48. EXERCISE 18: Flow Cytometry.- 49.EXERCISE 19: Complement Fixation Assays.- 50. Tissue Cell Culture Techniques.- 51. EXERCISE 20: Mitogen Induced Response of Lymphocytes:

Detection of Band T cell Lymphocytes Using Immunofluorescence.- 52. Exercise 21: Induction of IL-2 Secretion from Mouse Lymphoma Cells.- 53. EXERCISE 22: Adoptive Transfer of Lymphocytes: Band T cell Cooperation for Antibody Production.- 54. EXERCISE 23: Macrophage Migration Inhibition.- 55. EXERCISE 24: Monoclonal Antibody Preparation.- 56. EXERCISE 25: Immunization of mice with a recombinant protein.- 57. EXERCISE 26: Polarization of T helper cells into TH1 subset.- 58. Exercise 27: Immunoprecipitation.- 59. EXERCISE 28: Induction of apoptosis in T lymphocytes and detection of apoptotic markers.- 60. EXERCISE 29: Cloning and sequencing of cDNAs of mouse variable regions of H and L chains of monoclonal antibodies.- Appendix.- Sample Practice Questions.