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    Virology: A Laboratory Manual (Instructor's Manual)

    Virology: A Laboratory Manual (Instructor's Manual) by Wiedbrauk, Danny L.; Burleson, Florence G.; Chambers, Thomas M.;

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    Product details:

    • Publisher Elsevier Science
    • Date of Publication 2 October 1992

    • ISBN 9780121447311
    • Binding Paperback
    • No. of pages20 pages
    • Size 228x152 mm
    • Weight 60 g
    • Language English
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    Table of Contents:

    Introduction to the Virology Laboratory.
    Preparation of Cell Culture Medium.
    Cell Growth and Cell Passage.
    Counting Cells with a Hemacytometer.
    Primary Cell Cultures.
    Cryopreservation of Cell Lines.
    Virus Growth in Cell Culture.
    Virus Growth in Embroynated Eggs.
    TCID50.
    LD50.
    EID50 Assay.
    Plaque Assays.
    HA Assay.
    Hemadsorption.
    One-Step Growth Curve.
    Determination of Size.
    Thermostability.
    Sensitivity to Lipid Solvents.
    Sensitivity to low pH.
    Encephalomyocarditis Virus Neutralization.
    Production of Interferon.
    Plaque-Reduction Interferon Bioassay.
    Inhibition of Cytopathic Effect (CPE) Interferon Bioassay.
    Hemagglutination-Yield Reduction Bioassay.
    Isolation of Viral Nucleic Acids from Virions.
    Isolation of Viral Nucleic Acids from Infected Cells.
    Analysis of Viral Nucleic Acids by Gel Electrophoresis.

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