The Integrin Interactome
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| ISBN13: | 9781071609613 |
| ISBN10: | 10716096111 |
| Binding: | Hardback |
| No. of pages: | 315 pages |
| Size: | 254x178 mm |
| Weight: | 831 g |
| Language: | English |
| Illustrations: | 11 Illustrations, black & white; 43 Illustrations, color; 40 Tables, color |
| 264 |
This volume provides the most cutting edge technologies related to the study of integrin activation and the characterization of their vast interactomes. Chapters detail protocols on experimental approached to quantify focal adhesion parameters, integrin activation, and the lateral interaction of integrins with transmembrane binding partners. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, The Integrin Interactome: Methods and Protocols aims to give the reader a multi-scale journey from single bonds inside protein structures to the function of these crucial adhesion receptors at a whole organism level in physiology and pathology.
This volume provides the most cutting edge technologies related to the study of integrin activation and the characterization of their vast interactomes. Chapters detail protocols on experimental approached to quantify focal adhesion parameters, integrin activation, and the lateral interaction of integrins with transmembrane binding partners. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, The Integrin Interactome: Methods and Protocols aims to give the reader a multi-scale journey from single bonds inside protein structures to the function of these crucial adhesion receptors at a whole organism level in physiology and pathology.
1. Measurement of Integrin Activation and Conformational Changes on the Cell Surface by Soluble Ligand and Antibody Binding Assays
Zhengli Wang and Jieqing Zhu
2. Quantification of Integrin Activation and Ligation in Adherent Cells
Zaki Al-Yafeai and A. Wayne Orr
3. Multi-parametric Analysis of Focal Adhesions in Bi-Dimensional Substrates
Vanessa C. Talayero and Miguel Vicente-Manzanares
4. Focal Adhesion Isolation Assay using ECM Coated Magnetic Beads
Wesley Sturgess and Vinay Swaminathan
5. Functional Integrin Regulation through Interactions with Tetraspanin CD9
Álvaro Torres-Gómez, Beatriz Carde?es, Ester Díez-Sainz, Esther M. Lafuente, and Carlos Caba?as
6. Proximity-dependent Biotinylation (BioID) of Integrin Interaction Partners
Satu-Marja Myllymäki, Xiaonan Liu, Markku Varjosalo, and Aki Manninen
7. Analyzing the Integrin Adhesome by in situ Proximity Ligation Assay
Brian A. Perrino, Yeming Xie, and Cristina Alexandru
8. Single-Protein Tracking to Study Protein Interactions during integrin-based Migration
A.V. Radhakrishnan, Tianchi Chen, Filipe Nunes Vicente, Thomas Orré, Amine Mehidi, Olivier Rossier, and Grégory Giannone
9. Biochemical Characterization of the Integrin Interactome
Rejina B. Khan, Lorena Varela, Alana R. Cowell, and Benjamin T. Goult
10. Network Analysis of Integrin Adhesion Complexes
Frederic Li Mow Chee and Adam Byron
11. Surface Patterning for the Control of Receptor Clustering and Molecular Forces of Integrin-mediated Adhesions
Federica Pennarola and E. Ada Cavalcanti-Adam
12. Dynamics and Physics of Integrin Activation in Tumour Cells by Nano-Sized Extracellular Ligands and Electromagnetic Fields
Alkiviadis-Constantinos Cefalas, Vassilios Gavriil, Angelo Ferraro, Zoe Kollia, and Evangelia Sarantopoulou
13. Genetic Instruction of Megakaryocytes and Platelets Derived from Human Induced Pluripotent Stem Cells for Studies of Integrin Regulation
Ana Kasirer-Friede and Sanford J. Shattil
14. Quantitative Analysis of Integrin Trafficking
Enoir Farage and Patrick T. Caswell
15. Methods to Study Integrin Functions on Exosomes
Eiji Kawamoto, Eun Jeong Park, and Motomu Shimaoka
16. Functional Bioinformatics Analyses of the Matrisome and Integrin Adhesome
Edward Roy Horton
17. Quantifying Polarized Extracellular Matrix Secretion in Cultured Endothelial Cells
Fabiana Clapero, Dora Tortarolo, Donatella Valdembri, and Guido Serini
