The Integrin Interactome: Methods and Protocols

The Integrin Interactome

Methods and Protocols
 
Kiadás sorszáma: 1st ed. 2021
Kiadó: Humana
Megjelenés dátuma:
Kötetek száma: 1 pieces, Book
 
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A termék adatai:

ISBN13:9781071609613
ISBN10:10716096111
Kötéstípus:Keménykötés
Terjedelem:315 oldal
Méret:254x178 mm
Súly:831 g
Nyelv:angol
Illusztrációk: 11 Illustrations, black & white; 43 Illustrations, color; 40 Tables, color
272
Témakör:
Rövid leírás:

This volume provides the most cutting edge technologies related to the study of integrin activation and the characterization of their vast interactomes. Chapters detail protocols on experimental approached to quantify focal adhesion parameters, integrin activation, and the lateral interaction of integrins with transmembrane binding partners. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



 



Authoritative and cutting-edge, The Integrin Interactome: Methods and Protocols aims to give the reader a multi-scale journey from single bonds inside protein structures to the function of these crucial adhesion receptors at a whole organism level in physiology and pathology.

Hosszú leírás:

This volume provides the most cutting edge technologies related to the study of integrin activation and the characterization of their vast interactomes. Chapters detail protocols on experimental approached to quantify focal adhesion parameters, integrin activation, and the lateral interaction of integrins with transmembrane binding partners. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



 



Authoritative and cutting-edge, The Integrin Interactome: Methods and Protocols aims to give the reader a multi-scale journey from single bonds inside protein structures to the function of these crucial adhesion receptors at a whole organism level in physiology and pathology.


Tartalomjegyzék:

1. Measurement of Integrin Activation and Conformational Changes on the Cell Surface by Soluble Ligand and Antibody Binding Assays



Zhengli Wang and Jieqing Zhu



 



2. Quantification of Integrin Activation and Ligation in Adherent Cells



Zaki Al-Yafeai and A. Wayne Orr



 



3. Multi-parametric Analysis of Focal Adhesions in Bi-Dimensional Substrates



Vanessa C. Talayero and Miguel Vicente-Manzanares



 



4. Focal Adhesion Isolation Assay using ECM Coated Magnetic Beads



Wesley Sturgess and Vinay Swaminathan



 



5. Functional Integrin Regulation through Interactions with Tetraspanin CD9



Álvaro Torres-Gómez, Beatriz Carde?es, Ester Díez-Sainz, Esther M. Lafuente, and Carlos Caba?as



 



6. Proximity-dependent Biotinylation (BioID) of Integrin Interaction Partners



Satu-Marja Myllymäki, Xiaonan Liu, Markku Varjosalo, and Aki Manninen



 



7. Analyzing the Integrin Adhesome by in situ Proximity Ligation Assay



Brian A. Perrino, Yeming Xie, and Cristina Alexandru



 



8. Single-Protein Tracking to Study Protein Interactions during integrin-based Migration



A.V. Radhakrishnan, Tianchi Chen, Filipe Nunes Vicente, Thomas Orré, Amine Mehidi, Olivier Rossier, and Grégory Giannone



 



9. Biochemical Characterization of the Integrin Interactome



Rejina B. Khan, Lorena Varela, Alana R. Cowell, and Benjamin T. Goult



 



10. Network Analysis of Integrin Adhesion Complexes



Frederic Li Mow Chee and Adam Byron



 



11.       Surface Patterning for the Control of Receptor Clustering and Molecular Forces of Integrin-mediated Adhesions



Federica Pennarola and E. Ada Cavalcanti-Adam



 



12. Dynamics and Physics of Integrin Activation in Tumour Cells by Nano-Sized Extracellular Ligands and Electromagnetic Fields 



Alkiviadis-Constantinos Cefalas, Vassilios Gavriil, Angelo Ferraro, Zoe Kollia, and Evangelia Sarantopoulou



 



13. Genetic Instruction of Megakaryocytes and Platelets Derived from Human Induced Pluripotent Stem Cells for Studies of Integrin Regulation



Ana Kasirer-Friede and Sanford J. Shattil



 



14. Quantitative Analysis of Integrin Trafficking



Enoir Farage and Patrick T. Caswell



 



15. Methods to Study Integrin Functions on Exosomes



Eiji Kawamoto, Eun Jeong Park, and Motomu Shimaoka



 



16. Functional Bioinformatics Analyses of the Matrisome and Integrin Adhesome



Edward Roy Horton



 



17. Quantifying Polarized Extracellular Matrix Secretion in Cultured Endothelial Cells



Fabiana Clapero, Dora Tortarolo, Donatella Valdembri, and Guido Serini