Regulators and Effectors of Small GTPases, Part E: GTPases Involved in Vesicular Traffic

Long description:

Small GTPases play a key role in many aspects of contemporary cell biology: control of cell growth and differentiation; regulation of cell adhesion and cell movement; the organization of the actin cytoskeleton; and the regulation of intracellular vesicular transport.
This volume and its companions (Volumes 255, 256, 257, and the forthcoming 325) cover all biochemical and biological assays currently in use for analyzing the role of small GTPases in these aspects of cell biology at the molecular level.

Table of Contents:

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Section I. Rab GTPases

1: Structural Basis for Rab Function: An Overview

2: Expression of Wild-Type and Mutant Green Fluorescent Protein-Rabl for Fluorescence Microscopy Analysis

3: Fluorescence Methods for Monitoring Interactions of Rab Proteins with Nucleotides, Rab Escort Protein, and Geranylgeranyltransferase

4: Prenylation of Rab Proteins in Vitro by Geranylgeranyltransferases

5: Antibody and Oligonucleotide Probes to Distinguish Intracellular Expression and Localization Patterns of Rab GDP-Dissociation Inhibitor Isoforms

6: Expression, Purification, and Biochemical Properties of Ypt/Rab GTPase-Activating Proteins of Gyp Family

7: Purification and Properties of Rab3 GDP/GTP Exchange Protein

8: Purification and Properties of Rab3 GTPase-Activating Protein

9: Rabphilin-3: A Target Molecule for Rab3 Small G Proteins

10: Doc2α as Modulator of Ca2+-Dependent Exocytosis

11: Purification and Properties of a GTPase-Activating Protein for Yeast Rab GTPases

12: Purification and Characterization of Yeast Exocyst Complex

13: Expression and Properties of Rab4 and Its Effector Rabaptin-4 in Endocytic Recycling

14: Purification of EEA1 from Bovine Brain Cytosol Using Rab5 Affinity Chromatography and Activity Assays

15: Expression, Purification, and Characterization of Rab5 Effector Complex, Rabaptin-5/Rabex-5

16: Measurement of Rab5 Protein Kinase B/akt and Regulation of Ras-Activated Endocytosis

17: Expression, Purification, and Biochemical Properties of Rabkinesin-6 Domains and Their Interactions with Rab6A

18: Expression and Functional Analyses of Rab8 and Rablla in Exocytic Transport from trans-Golgi Network

19: Expression and Properties of Rab7 in Endosome Function

20: Expression, Purification, and Properties of Rab8 Function in Actin Cortical Skeleton Organization and Polarized Transport

21: Properties of Rab13 Interaction with Rod cGMP Phosphodiesterase Î ́ Subunit

22: Subcellular Localization of Rab17 by Cryo-Immunogold Electron Microscopy in Epithelial Cells Grown on Polycarbonate Filters

23: Expression and Properties of Rab25 in Polarized Madin-Darby Canine Kidney Cells

24: Purification of TRAPP from Saccharomyces cerevisiae and Identification of Its Mammalian Counterpart

Section II. ADP-Ribosylation Factor (ARF) GTPases

25: Structural and Functional Organization of ADP-Ribosylation Factor (ARF) Proteins

26: Expression and Properties of ADP-Ribosylation Factor (ARF6) in Endocytic Pathways

27: Expression and Analysis of ARNO and ARNO Mutants and Their Effects on ADP-Ribosylation Factor (ARF)-Mediated Actin Cytoskeletal Rearrangements

28: Expression, Purification, and Measurements of Activity of ARNO1, a Guanine Nucleotide Exchange Factor for ADP-Ribosylation Factor 1 (ARF1)

29: Expression, Purification, and Biochemical Properties of EFA6, a Sec7 Domain-Containing Guanine Exchange Factor for ADP-Ribosylation Factor 6 (ARF6)

30: Isolation and Properties of GRP1, an ADP-Ribosylation Factor (ARF)-Guanine Nucleotide Exchange Protein Regulated by Phosphatidylinositol 3, 4, 5-Trisphosphate

31: Functional Analysis of ADP-Ribosylation Factor (ARF) Guanine Nucleotide Exchange Factors Gea1p and Gea2p in Yeast

32: Isolation, Cloning, and Characterization of Brefeldin A-Inhibited Guanine Nucleotide-Exchange Protein for ADP-Ribosylation Factor

33: Expression, Purification, and Properties of ADP-Ribosylation Factor (ARF) GTPase Activating Protein-1

34: Expression, Analysis, and Properties of Yeast ADP-Ribosylation Factor (ARF) GTPase Activating Proteins (GAPs) Gcs1 and Glo3

35: Purification and Properties of ARD1, An ADP-Ribosylation Factor (ARF)-Related Protein with GTPase-Activating Domain

36: Purification and Characterization of GIT Family of ADP-Ribosylation Factor (ARF) GTPase-Activating Proteins

37: Assay and Purification of Phosphoinositide-Dependent ADP-Ribosylation Factor (ARF) GTPase Activating Proteins

38: Biological Properties and Measurement of Phospholipase D Activation by ADP-Ribosylation Factor (ARF)

39: Use of Aminoglycoside Antibiotics and Related Compounds to Study ADP-Ribosylation Factor (ARF) /Coatomer Function in Golgi Traffic

40: Adaptor Protein 1-Dependent Clathrin Coat Assembly on Synthetic Liposomes and Golgi Membranes

41: Receptor-Dependent Formation of COPI-Coated Vesicles from Chemically Defined Donor Liposomes

42: ADP-Ribosylation Factor (ARF) as Regulator of Spectrin Assembly at Golgi Complex

43: Purification, Properties, and Analysis of yARL3

44: Preparation and Assay of Recombinant ADP-Ribosylation Factor-Like Protein-1 (ARL1)

Section III. Sar GTPases

45: Purification and Properties of Rat Liver Sec23ï¿1⁄2?""Sec24 Complex

46: Purification of Functional Sec13pï¿1⁄2?""Sec31p Complex, A Subunit of COPII Coat

Section IV. Dynamin GTPases

47: Expression, Purification, and Functional Assays for Self-Association of Dynamin-1

48: Analysis of Phosphoinositide Binding by Pleckstrin Homology Domain from Dynamin

49: Mapping Dynamin Interdomain Interactions with Yeast Two-Hybrid and Glutathione S-Transferase Pulldown Experiments

50: Interactions of Dynamin and Amphiphysin with Liposomes

51: Activation of Dynamin GTPase Activity by Phosphoinositides and SH3 Domain-Containing Proteins

Section V. Septin GTPases

52: Expression and Analysis of Properties of Septin CDCrel-1 in Exocytosis

Author Index

Subject Index