| ISBN13: | 9781071610947 |
| ISBN10: | 1071610945 |
| Binding: | Hardback |
| No. of pages: | 297 pages |
| Size: | 254x178 mm |
| Weight: | 787 g |
| Language: | English |
| Illustrations: | 14 Illustrations, black & white; 51 Illustrations, color; 52 Tables, color |
| 0 |
Eosinophils
EUR 192.59
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This second edition of Eosinophils: Methods and Protocols updates several techniques from the first edition together with novel in vitro and in vivo-based methodologies. Written by internationally recognized expert authorities, this volume provides vital techniques from eosinophil purification to experimental modelling, with each technique spelled out in clear and straightforward terms, assuming no previous knowledge of the method and without necessitating sourcing additional information elsewhere. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the essential materials and reagents, step-by-step, readily reproducible laboratory protocols, with tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Eosinophils: Methods and Protocols, Second Edition provides the practical means to extend our knowledge of eosinophil function in health and disease, underpinning research that may in turn lead to new hypotheses for future examinations into the role of this intriguing and enigmatic leukocyte.
Chapters 10 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Chapters 10 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Chapters 10 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
1. Molecular Biology of Eosinophils: Introduction
Paige Lacy, Helene F. Rosenberg, and Garry M. Walsh
2. Functionally Active Eosinophil Purification from Peripheral Blood
Kiho Son, Paige Lacy, and Manali Mukherjee
3. Eosinophil Purification from Peripheral Blood of Rhesus Monkeys
Fanny Legrand
4. Generation of Mouse Eosinophils in Tissue Culture from Unselected Bone Marrow Progenitors
Eric Mai, Ajinkya R. Limkar, Caroline M. Percopo, and Helene F. Rosenberg
5. Detection of Mouse Eosinophils in Tissue by Flow Cytometry and Isolation by Fluorescence
-Activated Cell Sorting (FACS)
Caroline M. Percopo, Ajinkya R. Limkar, Albert C. Sek, and Helene F. Rosenberg
6. Mutant Mice and Animal Models of Airway Allergic Disease
Julyanne Brassard, David Marsolais, and Marie
-Renee Blanchet
7. Eosinophils in Asthma Models to House Dust Mite for Drug Development
François Daubeuf and Nelly Frossard
8. Analysis of Mouse Eosinophil Migration and Killing of Tumor Cells
Sharon Grisaru, Michal Itan, and Ariel Munitz
9. Murine Models of Eosinophil Function in Fungal and Viral Infections
Meenakshi Tiwary, Kim S. LeMessurier, and Amali E. Samarasinghe
10. Assays of Eosinophil Apoptosis and Phagocytic Uptake
Naomi N. Gachanja, David A. Dorward, Adriano G. Rossi, and Christopher D. Lucas
11. In Vivo Eosinophil Expansion Using Gene Transfer by Electroporation
Emi Hamaguchi
-Tsuru, Masataka Korenaga, and Fabrizio Bruschi
12. Observation and Quantification of Eosinophil Motility
Karina T. Barretto, Deane F. Mosher, Nizar N. Jarjour, Colleen S. Curran, Stephane Esnault, and Mats W. Johansson
13. Isolation and Functional Aspects of Eosinophil
-Derived Exosomes
José Antonio Ca?as, José Manuel Rodrigo
-Mu?oz, and Victoria del Pozo
14. Interactions of Eosinophils with Nerves
Matthew G. Drake, Becky J. Proskocil, and Zhenying Nie
15. Measurement of Eosinophil Kinetics In Vivo
Neda Farahi, Daniel Gillett, Chrystalla Loutsios, A. Michael Peters, Charlotte Summers, and Edwin R. Chilvers
16. Detection of Eosinophil Extracellular DNA Traps
Marina Valente Barroso and Josiane S. Neves
17. Eosinophil Shape Change and Secretion
Sergei I. Ochkur, Elizabeth A. Jacobsen, and Paige Lacy
18. Update on Eosinophils Interaction with Mast Cells: The Allergic Effector Unit
Roopesh Singh Gangwar, Hadas Pahima, Pier Giorgio Puzzovio, and Francesca Levi
-Schaffer
19. Assessing Phenotypic Heterogeneity in Intestinal Tissue Eosinophils
Courtney L. Olbrich, Leigha D. Larsen, and Lisa A. Spencer
20. Antimicrobial Activity of Human Eosinophil Granule Proteins
Anu Chopra and Janendra K. Batra
21. Experimental Modeling of Eosinophil
-Associated Diseases
Sathisha Upparahalli Venkateshaiah, Murli Manohar, Hemanth Kumar Kandikattu, and Anil Mishra
