Eosinophils: Methods and Protocols

Eosinophils

Methods and Protocols
 
Kiadás sorszáma: 2nd ed. 2021
Kiadó: Humana
Megjelenés dátuma:
Kötetek száma: 1 pieces, Book
 
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EUR 192.59
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A termék adatai:

ISBN13:9781071610947
ISBN10:1071610945
Kötéstípus:Keménykötés
Terjedelem:297 oldal
Méret:254x178 mm
Súly:787 g
Nyelv:angol
Illusztrációk: 14 Illustrations, black & white; 51 Illustrations, color; 52 Tables, color
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Rövid leírás:

This second edition of Eosinophils: Methods and Protocols updates several techniques from the first edition together with novel in vitro and in vivo-based methodologies. Written by internationally recognized expert authorities, this volume provides vital techniques from eosinophil purification to experimental modelling, with each technique spelled out in clear and straightforward terms, assuming no previous knowledge of the method and without necessitating sourcing additional information elsewhere. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the essential materials and reagents, step-by-step, readily reproducible laboratory protocols, with tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Eosinophils: Methods and Protocols, Second Edition provides the practical means to extend our knowledge of eosinophil function in health and disease, underpinning research that may in turn lead to new hypotheses for future examinations into the role of this intriguing and enigmatic leukocyte.

Chapters 10 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Hosszú leírás:
This second edition of Eosinophils: Methods and Protocols updates several techniques from the first edition together with novel in vitro and in vivo-based methodologies. Written by internationally recognized expert authorities, this volume provides vital techniques from eosinophil purification to experimental modelling, with each technique spelled out in clear and straightforward terms, assuming no previous knowledge of the method and without necessitating sourcing additional information elsewhere. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the essential materials and reagents, step-by-step, readily reproducible laboratory protocols, with tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Eosinophils: Methods and Protocols, Second Edition provides the practical means to extend our knowledge of eosinophil function in health and disease, underpinning research that may in turn lead to new hypotheses for future examinations into the role of this intriguing and enigmatic leukocyte.

Chapters 10 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Tartalomjegyzék:

1. Molecular Biology of Eosinophils: Introduction



            Paige Lacy, Helene F. Rosenberg, and Garry M. Walsh



 



2. Functionally Active Eosinophil Purification from Peripheral Blood



            Kiho Son, Paige Lacy, and Manali Mukherjee



 



3. Eosinophil Purification from Peripheral Blood of Rhesus Monkeys



            Fanny Legrand



 



4. Generation of Mouse Eosinophils in Tissue Culture from Unselected Bone Marrow Progenitors



            Eric Mai, Ajinkya R. Limkar, Caroline M. Percopo, and Helene F. Rosenberg



 



5. Detection of Mouse Eosinophils in Tissue by Flow Cytometry and Isolation by Fluorescence
-Activated Cell Sorting (FACS)



            Caroline M. Percopo, Ajinkya R. Limkar, Albert C. Sek, and Helene F. Rosenberg



 



6. Mutant Mice and Animal Models of Airway Allergic Disease



            Julyanne Brassard, David Marsolais, and Marie
-Renee Blanchet



 



7. Eosinophils in Asthma Models to House Dust Mite for Drug Development



            François Daubeuf and Nelly Frossard



 



8. Analysis of Mouse Eosinophil Migration and Killing of Tumor Cells



            Sharon Grisaru, Michal Itan, and Ariel Munitz



 



9. Murine Models of Eosinophil Function in Fungal and Viral Infections



            Meenakshi Tiwary, Kim S. LeMessurier, and Amali E. Samarasinghe



 



10. Assays of Eosinophil Apoptosis and Phagocytic Uptake



            Naomi N. Gachanja, David A. Dorward, Adriano G. Rossi, and Christopher D. Lucas



 



11. In Vivo Eosinophil Expansion Using Gene Transfer by Electroporation



            Emi Hamaguchi
-Tsuru, Masataka Korenaga, and Fabrizio Bruschi



 



12. Observation and Quantification of Eosinophil Motility



            Karina T. Barretto, Deane F. Mosher, Nizar N. Jarjour, Colleen S. Curran, Stephane Esnault, and Mats W. Johansson



 



13. Isolation and Functional Aspects of Eosinophil
-Derived Exosomes



            José Antonio Ca?as, José Manuel Rodrigo
-Mu?oz, and Victoria del Pozo



 



14. Interactions of Eosinophils with Nerves



            Matthew G. Drake, Becky J. Proskocil, and Zhenying Nie



 



15. Measurement of Eosinophil Kinetics In Vivo



            Neda Farahi, Daniel Gillett, Chrystalla Loutsios, A. Michael Peters, Charlotte Summers, and Edwin R. Chilvers



 



16. Detection of Eosinophil Extracellular DNA Traps



            Marina Valente Barroso and Josiane S. Neves



 



17. Eosinophil Shape Change and Secretion



            Sergei I. Ochkur, Elizabeth A. Jacobsen, and Paige Lacy



 



18. Update on Eosinophils Interaction with Mast Cells: The Allergic Effector Unit



            Roopesh Singh Gangwar, Hadas Pahima, Pier Giorgio Puzzovio, and Francesca Levi
-Schaffer



 



19. Assessing Phenotypic Heterogeneity in Intestinal Tissue Eosinophils



            Courtney L. Olbrich, Leigha D. Larsen, and Lisa A. Spencer



 



20. Antimicrobial Activity of Human Eosinophil Granule Proteins



            Anu Chopra and Janendra K. Batra



 



21. Experimental Modeling of Eosinophil
-Associated Diseases



            Sathisha Upparahalli Venkateshaiah, Murli Manohar, Hemanth Kumar Kandikattu, and Anil Mishra