Hepatitis C Virus Protocols
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| ISBN13: | 9781493989751 |
| ISBN10: | 1493989758 |
| Kötéstípus: | Keménykötés |
| Terjedelem: | 508 oldal |
| Méret: | 254x178 mm |
| Súly: | 1198 g |
| Nyelv: | angol |
| Illusztrációk: | 28 Illustrations, black & white; 47 Illustrations, color |
| 74 |
This volume details the most updated concepts and experimental protocols developed by leading researchers in the field. Chapters guide readers through methods on bioinformatics tools, hepatitis c virus(HCV) cloning, culture, and purification, HCV life cycle, host immune responses, and small animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Hepatitis C Virus Protocols aims to ensure successful results in the further study of this vital field.
This volume details the most updated concepts and experimental protocols developed by leading researchers in the field. Chapters guide readers through methods on bioinformatics tools, hepatitis c virus(HCV) cloning, culture, and purification, HCV life cycle, host immune responses, and small animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Hepatitis C Virus Protocols aims to ensure successful results in the further study of this vital field.
Part I: Review of Hepatitis C Virus and Bioinformatic Tools
1. Overview of Direct Acting Antiviral Drugs and Drug Resistance of Hepatitis C Virus
Darrick K. Li and Raymond T. Chung
2. Similarities and Differences between HCV Pseudoparticle (HCVpp) and Cell Culture HCV (HCVcc) in the Study of HCV
Laura Riva and Jean Dubuisson
3. Hepatitis C Virus Database and Bioinformatics Analysis Tools in the Virus Pathogen Resource (ViPR)
Yun Zhang, Christian Zmasek, Guangyu Sun, Christopher N. Larsen, and Richard H. Scheuermann
Part II: Methods for Virus Cloning, Culture, and Purification
4. Establishment of Replication-competent HCV Strain with Minimum Modifications
Takanobu Kato, Asako Murayama, and Takaji Wakita
5. Full-length Open Reading Frame Amplification of Hepatitis C Virus
Ulrik Fahn?e and Jens Bukh
6. InFusion Cloning for the Generation of Biologically Relevant HCV Chimeric Molecular Clones
Barnabas King, Richard Urbanowicz, Alexander W. Tarr, Jonathan K. Ball, and C. Patrick McClure
7. Production and Purification of Cell Culture Hepatitis C Virus
Cynthia de la Fuente and Maria Teresa Catanese
8. Embryonic or Induced Pluripotent Stem Cell-derived Hepatocellular Systems for HCV Culture
Xianfang Wu and Viet Loan Dao Thi
Part III: Methods for the Study of HCV Life Cycle
9. Molecular Identification of Transmitted/Founder Hepatitis C Viruses and their Progeny by Single Genome Sequencing
Hui Li, Muhammad N. Zahid, Shuyi Wang, and George M. Shaw
10. A Cell Culture Model for Persistent HCV Infection
Victoria Castro, Ginés Ávila, Lidia Mingorance, and Pablo Gastaminza
11. Expression Cloning of Host Factors Required for the HCV Replication Cycle
Sandra Ciesek and Thomas von Hahn
12. Application of Deep Mutational Scanning in Hepatitis C Virus
Nicholas C. Wu and Hangfei Qi
13. Studying the Hepatitis C Virus-induced Epigenetic Signature after Cure with Direct-acting Antivirals
Shira Perez and Meital Gal-Tanamy
14. Measuring Hepatitis C Virus Envelopment by using a Proteinase K Protection Assay
Allison E. Roder and Stacy M. Horner
15. Membrane Fusion Assays for Studying Entry Hepatitis C Virus into Cells
Sol?ne Denolly, François-Lo?c Cosset, and Natalia Freitas
16. Functional Characterization of Apolipoproteins in the HCV Life Cycle
Luhua Qiao and Guangxiang (George) Luo
17. Investigating Hepatitis C Virus Infection Using Super-resolution Microscopy
Pedro Matos Pereira, Caron Jacobs, and Joe Grove
18. Live Cell Imaging of Hepatitis C Virus Trafficking in Hepatocytes
Yasmine Baktash and Glenn Randall
19. Cloning and Analysis of Authentic Patient-derived HCV E1/E2 Glycoproteins
Richard A. Urbanowicz, Jonathan K. Ball, and Alexander W. Tarr
20. Simultaneous Quantification of Hepatitis C virus Envelope Glycoproteins E1 and E2 by Dual-color Fluorescence Immunoblot Analysis
Fang Chen, Erick Giang, Radhika Gopal, and Mansun Law
21. Overcoming Challenges of Hepatitis C Virus Envelope Glycoprotein Production in Mammalian Cells
Samantha A. Yost, Jillian Whidby, Abdul G. Khan, Yuanyuan Wang, and Joseph Marcotrigiano
Part IV: Methods for Studying Immune Responses
22. Monitoring of Interferon Response Triggered by Cells Infected by Hepatitis C Virus or Other Viruses upon Cell-cell Contact
Séverin Coléon, Sonia Assil, and Marl?ne Dreux
23. Complement Regulation and Immune Evasion by Hepatitis C Virus
Young-Chan Kwon and Ranjit Ray
24. Direct ex vivo Functional Analysis of HCV-specific T Cells
Su-Hyung Park and Eui-Cheol Shin
25. Simultaneous Analysis of HCV-specific CD4+ and CD8+ T Cells by Multi-color Flow Cytometry
David Wolski and Georg M Lauer
26. Probing the Antigenicity of HCV Envelope Glycoproteins by Phage Display Antibody Technology
Erick Giang, Fernando Aleman, and Mansun Law
27. Isolation of HCV Neutralizing Antibodies by Yeast Display
Zhenyong Keck, Yong Wang, Patrick Lau, and Steven KH Foung
28. Detection of Antibodies to HCV E1E2 by Lectin-Capture ELISA
Marian Major and Mansun Law
29. In vitro Neutralization Assay using Cultured Hepatitis C Virus
Jannick Prentoe and Jens Bukh
30. Standardized Method for the Study of Antibody Neutralization of HCV pseudoparticles (HCVpp)
Justin R. Bailey, Richard A. Urbanowicz, Jonathan K. Ball, Mansun Law, and Steven K. H. Foung
Part V: Small Animal Models
31. Detection of Antigen-specific CD8+ T Cells in the Livers of HCV Core Transgenic Mice
Dustin A. Cobb, Aditya P. Dandekar, and Young S. Hahn
32. Mice with Chimeric Human Livers and Their Applications
Donna N Douglas and Norman M Kneteman
33. Mouse Models for Studying HCV Vaccines and Therapeutic Antibodies
Jenna M. Gaska, Qiang Ding, and Alexander Ploss
