Hepatitis C Virus Protocols

Hepatitis C Virus Protocols

 
Edition number: 1st ed. 2019
Publisher: Humana
Date of Publication:
Number of Volumes: 1 pieces, Book
 
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Product details:

ISBN13:9781493989751
ISBN10:1493989758
Binding:Hardback
No. of pages:508 pages
Size:254x178 mm
Weight:1198 g
Language:English
Illustrations: 28 Illustrations, black & white; 47 Illustrations, color
549
Category:
Short description:

This volume details the most updated concepts and experimental protocols developed by leading researchers in the field. Chapters guide readers through methods on bioinformatics tools, hepatitis c virus(HCV) cloning, culture, and purification, HCV life cycle, host immune responses, and small animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Hepatitis C Virus Protocols aims to ensure successful results in the further study of this vital field.

Long description:

This volume details the most updated concepts and experimental protocols developed by leading researchers in the field. Chapters guide readers through methods on bioinformatics tools, hepatitis c virus(HCV) cloning, culture, and purification, HCV life cycle, host immune responses, and small animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Hepatitis C Virus Protocols aims to ensure successful results in the further study of this vital field.

Table of Contents:

Part I: Review of Hepatitis C Virus and Bioinformatic Tools



1. Overview of Direct Acting Antiviral Drugs and Drug Resistance of Hepatitis C Virus



Darrick K. Li and Raymond T. Chung



 



2. Similarities and Differences between HCV Pseudoparticle (HCVpp) and Cell Culture HCV (HCVcc) in the Study of HCV



Laura Riva and Jean Dubuisson



 



3. Hepatitis C Virus Database and Bioinformatics Analysis Tools in the Virus Pathogen Resource (ViPR)



Yun Zhang, Christian Zmasek, Guangyu Sun, Christopher N. Larsen, and Richard H. Scheuermann



 



Part II: Methods for Virus Cloning, Culture, and Purification



4. Establishment of Replication-competent HCV Strain with Minimum Modifications



Takanobu Kato, Asako Murayama, and Takaji Wakita



 



5. Full-length Open Reading Frame Amplification of Hepatitis C Virus



Ulrik Fahnoe and Jens Bukh



 



6. InFusion Cloning for the Generation of Biologically Relevant HCV Chimeric Molecular Clones



Barnabas King, Richard Urbanowicz, Alexander W. Tarr, Jonathan K. Ball, and C. Patrick McClure



 



7. Production and Purification of Cell Culture Hepatitis C Virus



Cynthia de la Fuente and Maria Teresa Catanese



 



8. Embryonic or Induced Pluripotent Stem Cell-derived Hepatocellular Systems for HCV Culture



Xianfang Wu and Viet Loan Dao Thi



 



 



Part III: Methods for the Study of HCV Life Cycle



9. Molecular Identification of Transmitted/Founder Hepatitis C Viruses and their Progeny by Single Genome Sequencing



Hui Li, Muhammad N. Zahid, Shuyi Wang, and George M. Shaw



 



10. A Cell Culture Model for Persistent HCV Infection



Victoria Castro, Ginés Ávila, Lidia Mingorance, and Pablo Gastaminza



 



11. Expression Cloning of Host Factors Required for the HCV Replication Cycle



Sandra Ciesek and Thomas von Hahn



 



12. Application of Deep Mutational Scanning in Hepatitis C Virus



Nicholas C. Wu and Hangfei Qi



 



13. Studying the Hepatitis C Virus-induced Epigenetic Signature after Cure with Direct-acting Antivirals



Shira Perez and Meital Gal-Tanamy



14. Measuring Hepatitis C Virus Envelopment by using a Proteinase K Protection Assay



Allison E. Roder and Stacy M. Horner



 



15. Membrane Fusion Assays for Studying Entry Hepatitis C Virus into Cells



Solene Denolly, François-Loic Cosset, and Natalia Freitas



 



16. Functional Characterization of Apolipoproteins in the HCV Life Cycle



Luhua Qiao and Guangxiang (George) Luo



17. Investigating Hepatitis C Virus Infection Using Super-resolution Microscopy



Pedro Matos Pereira, Caron Jacobs, and Joe Grove



 



18. Live Cell Imaging of Hepatitis C Virus Trafficking in Hepatocytes

Yasmine Baktash and Glenn Randall



 



19. Cloning and Analysis of Authentic Patient-derived HCV E1/E2 Glycoproteins



Richard A. Urbanowicz, Jonathan K. Ball, and Alexander W. Tarr



 



20. Simultaneous Quantification of Hepatitis C virus Envelope Glycoproteins E1 and E2 by Dual-color Fluorescence Immunoblot Analysis



Fang Chen, Erick Giang, Radhika Gopal, and Mansun Law



 



21. Overcoming Challenges of Hepatitis C Virus Envelope Glycoprotein Production in Mammalian Cells



Samantha A. Yost, Jillian Whidby, Abdul G. Khan, Yuanyuan Wang, and Joseph Marcotrigiano



 



Part IV: Methods for Studying Immune Responses



22. Monitoring of Interferon Response Triggered by Cells Infected by Hepatitis C Virus or Other Viruses upon Cell-cell Contact



Séverin Coléon, Sonia Assil, and Marlene Dreux



 



23. Complement Regulation and Immune Evasion by Hepatitis C Virus



Young-Chan Kwon and Ranjit Ray



 



24. Direct ex vivo Functional Analysis of HCV-specific T Cells



Su-Hyung Park and Eui-Cheol Shin



 



25. ­Simultaneous Analysis of HCV-specific CD4+ and CD8+ T Cells by Multi-color Flow Cytometry

David Wolski and Georg M Lauer



 



26. Probing the Antigenicity of HCV Envelope Glycoproteins by Phage Display Antibody Technology



Erick Giang, Fernando Aleman, and Mansun Law



27. Isolation of HCV Neutralizing Antibodies by Yeast Display



Zhenyong Keck, Yong Wang, Patrick Lau, and Steven KH Foung



 



28. Detection of Antibodies to HCV E1E2 by Lectin-Capture ELISA



Marian Major and Mansun Law



 



29. In vitro Neutralization Assay using Cultured Hepatitis C Virus



Jannick Prentoe and Jens Bukh



 



30. Standardized Method for the Study of Antibody Neutralization of HCV pseudoparticles (HCVpp)



Justin R. Bailey, Richard A. Urbanowicz, Jonathan K. Ball, Mansun Law, and Steven K. H. Foung



 



Part V: Small Animal Models



31. Detection of Antigen-specific CD8+ T Cells in the Livers of HCV Core Transgenic Mice



Dustin A. Cobb, Aditya P. Dandekar, and Young S. Hahn



 



32. Mice with Chimeric Human Livers and Their Applications



Donna N Douglas and Norman M Kneteman



 



33. Mouse Models for Studying HCV Vaccines and Therapeutic Antibodies



Jenna M. Gaska, Qiang Ding, and Alexander Ploss