Spectrophotometry and Spectrofluorimetry
A Practical Approach
Series: Practical Approach Series; 225;
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Product details:
- Edition number 2
- Publisher OUP Oxford
- Date of Publication 4 May 2000
- ISBN 9780199638123
- Binding Paperback
- No. of pages386 pages
- Size 246x189x23 mm
- Weight 763 g
- Language English
- Illustrations numerous figures 0
Categories
Short description:
Spectrophotometry and Spectrofluorimetry: A Practical Approach Second Edition describes the theory and practical applications of many analytical techniques based upon the light absorbing properties of molecules. Such techniques form the basis of routine tests in chemical, biological and medical science laboratories. Practical advice is given, from experts in the different topics, together with lists of useful literature.
MoreLong description:
Spectrophotometry and Spectrofluorimetry: A Practical Approach Second Edition was written with the intention to help the reader understand the background concepts and practical applications of spectrophotometry and spectrofluorimetry. Optical spectroscopy underpins the day to day operations of most laboratories in the chemical, biological and medical sciences and this edition contains substantially updated and new chapters addressing the principles of most of the more common applications such as: spectrophotometry, spectrophotometric assays, spectrofluorimetry, time resolved fluorescence and phosphorescence studies, circular dichrosim and pre-equilibrium spectroscopic techniques. In all chapters, the emphasis is placed upon the practical aspects, with protocols to guide readers through test experiments. Other chapters are included to introduce subjects that have traditionally depended upon spectroscopy such as basic enzyme kinetics, ligand binding, data handling and the more recently established interest in the study of protein and DNA stability. Finally, the concept of 'global analysis' is introduced to provide the reader with an insight into this method of utilizing the vast arrays of experimental data provided by current instrumentation.
'...Practical advice is given by experts in the different topics, together with lists of useful literature'Librairie
Table of Contents:
Introduction to light absorption; visible and ultraviolet spectra
Fluorescence principles and measurement
Time resolved fluorescence and phosphorescence spectroscopy
An introduction to circular dichroism
Quantitative determination of equilibrium binding isotherms for the multiple ligand - macromolecule interactions using spectroscopic methods
Steady state kinetics
Spectrophotometric assays
Stopped flow spectroscopy
Stopped flow fluorescence
Stopped flow circular dichroism
Spectrophotometry and fluorimetry of cellular compartments and intracellular processes
Use of optical spectroscopic methods to study the thermodynamic stability of proteins
The use of spectroscopic techniques in the study of protein stability
