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Product details:
- Edition number 3
- Publisher OUP Oxford
- Date of Publication 1 October 1998
- ISBN 9780199636402
- Binding Paperback
- No. of pages372 pages
- Size 236x158x20 mm
- Weight 535 g
- Language English
- Illustrations halftones, line drawings 0
Categories
Short description:
Gel Electrophoresis of Proteins: A Practical Approach (third edition) is an up-to-date manual providing details of a variety of methods for running protein gels that will be very widely applicable and an abundance of new techniques and equipment developed since the publication of the second edition. As with the highly successful second edition, this new text places an emphasis on the practical aspects involved in the techniques - presenting them in a clear and accessible format.
MoreLong description:
This new edition of Gel Electrophoresis of Proteins is a completely new text, with eight of the ten chapters written by new authors. It presents the best methods, hints and tips for core procedures such as one- dimensional polyacrylamide gel electrophoresis, isoelectric focusing, two-dimensional gel electrophoresis, preparative gel electrophoresis, and peptide mapping, complete with the latest refinements and updates of the procedures. In addition, it describes major new techniques which have come to the fore since the previous edition. Thus there are chapters on capillary gel electrophoresis, sequence analysis of gel-resolved proteins, fluorophore-labelled saccharide electrophoresis, and analysis of protein:protein interactions by gel electrophoresis. One thing has not changed. The emphasis is still on describing the best methods, in step-by-step detail, with copious advice to ensure that each method works first time in the reader's hands. The first two editions of Gel Electrophoresis of Proteins: A Practical Approach each gained a strong reputation as easy-to-follow laboratory manuals written by experienced researchers for researchers. The methods were presented in a clear accessible format and had been fully tested to ensure success in the lab. This new edition will strengthen the reputation of the book still further. It is a 'must have' for all those who currently use gel electrophoresis or who plan to do so.
MoreTable of Contents:
One-dimensional PAGE
Protein detection
Preparative gel electrophoresis
Capillary SDS electrophoresis
Conventional isoelectric focusing
2-D gel electrophoresis
Peptide mapping
Sequence analysis of gel-resolved proteins
Analysis of glycoproteins
Protein: protein interactions
Appendix 1 - List of suppliers
Appendix 2 - Bibliography of polypeptide detection methods
