G Protein-Coupled Receptor Genetics by Stevens, Craig W.;

Long description:

G Protein‐Coupled Receptor Genetics: Research and Methods in the Post‐Genomic Era features practical techniques inspired by the fast moving GPCR field. From powerful bioinformatic tools tracing the evolution of GPCRs, to methods for the cellular transfection of engineered viruses containing GPCRs, to optogenetic techniques that produce light-activated GPCRs in live mice, what was once science fiction is now science fact. This detailed volume includes sections covering genetic mechanisms, a genetic toolbox for GPCR discovery, as well as genetic aspects of G protein-coupled receptors in health and medicine. Written for the Methods in Pharmacology and Toxicology series, this book contains the kind of key implementation advice that encourages successful results in the lab.

Authoritative and easy to use, G Protein‐Coupled Receptor Genetics: Research and Methods in the Post‐Genomic Era serves as an ideal guide for researchers aiming to continue our progress in this dynamic and exciting area of study.

Table of Contents:

G Protein-Coupled Receptors: Research and Methods in the Post-Genomic Era.- Examining the Dynamic Evolution of G Protein-Coupled Receptors.- Functional Properties of Virus-Encoded and Virus-Regulated G Protein-Coupled Receptors.- Discovery and Function of the Very Large G Protein-Coupled Receptor.- Functional Evolution of Opioid Family G Protein-Coupled Receptors.- Investigation of G Protein-Coupled Receptor Function and Regulation Using Antisense.- Signalosome Profiling Reveals Allosteric Interactions Between G Protein-Coupled Receptors.- Use of Optogenetic Approaches to Control Intracellular Signaling of G Protein-Coupled Receptors.- Viral Infection for G Protein-Coupled Receptor Expression in Eukaryotic Cells.- Using In Vitro Mutagenesis to Characterize Structure-Function Relationships in G Protein-Coupled Receptors.- The CRE Luc Mouse Model for Bioimaging Ligand Activation of G Protein-Coupled Receptors.- Inducing Conformational Changes in G Protein-Coupled Receptors by Domain Coupling.- Rescue of Defective G Protein-Coupled Receptor Function by Intermolecular Cooperation.- Design of Super-Arrestins for Gene Therapy of Diseases Associated with Excessive Signaling of G Protein-Coupled Receptors.- Importance of G Protein-Coupled Receptor Genetics in Clinical Medicine.- Pharmacogenomics of G Protein-Coupled Receptor Signaling and Other Pathways in Essential Hypertension.- Exon Sequencing of G Protein-Coupled Receptor Genes and Perspectives for Disease Treatment.- Gene Therapy Using G Protein-Coupled Receptors for the Treatment of Cardiovascular Disease.- Novel Mechanisms of G Protein-Coupled Receptor Oligomer and Ion Channel Interactions in Nociception.- Discovery and Characterization of Leucine-Rich Repeat-Containing G Protein-Coupled Receptors.- The Role of G-Protein Coupled Receptors in Adult Neurogenesis.