Essential Molecular Biology Vol 2
Series: Practical Approach Series; 255;
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Product details:
- Edition number 2
- Publisher Oxford University Press
- Date of Publication 15 November 2001
- ISBN 9780199636440
- Binding Paperback
- No. of pages310 pages
- Size 248x190x18 mm
- Weight 613 g
- Language English
- Illustrations 11 line drawings, 9 halftones 0
Categories
Short description:
The two Essential Molecular Biology books in the Practical Approach Series are designed for the absolute beginner at gene cloning whether they be at the start of their career or an experienced researcher in another field. As with the first editions, the objective of both volumes is to combine solid practical information with sufficient background material to ensure that the novice can understand how a technique works, what it achieves, and how to make
modifications to suit personal requirements.
Long description:
The two Essential Molecular Biology books in the Practical Approach Series are designed for the absolute beginner at gene cloning whether they be at the start of their career or an experienced researcher in another field. As with the first editions, the objective of both volumes is to combine solid practical information with sufficient background material to ensure that the novice can understand how a technique works, what it achieves, and how to make modifications
to suit personal requirements.
Volume 2 details procedures for isolating and studying individual genes (preparation and screening of libraries, polymerase chain reactions, DNA sequencing and studying gene expression). It is assumed that the basics of Volume 1 are now in place, but the procedures are still described in the same down to earth fashion with protocols complemented by background information and troubleshooting hints.
Table of Contents:
Strategies for research in molecular biology
Construction of genomic libraries in lambda and cosmic vectors
Construction of a cDNA library
Nucleic acid labelling and detection
Immobilization of nucleic acids and hybridization analysis
DNA sequencing
The polymerase chain reaction
Protein expression in Escherichia coli
Transcript mapping
Health hazards and safety procedures
Equipment for molecular biology research
Important Escherichia coli strains
Recipes and general procedures
Restriction endonucleases
DNA and RNA modification enzymes
List of suppliers' addresses
