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Product details:
- Edition number 3
- Publisher OUP Oxford
- Date of Publication 29 June 2000
- ISBN 9780199637966
- Binding Paperback
- No. of pages334 pages
- Size 247x189x20 mm
- Weight 655 g
- Language English
- Illustrations 15 halftones, numerous line figures 0
Categories
Short description:
Animal cell culture is an essential feature of most biological and medical research. This volume provides all those who use cell culture with advice, information, and above all practical guidance on methods. A copy of the third edition of Animal Cell Culture should be available in every laboratory.
MoreLong description:
This new edition of Animal Cell Culture covers new or updated chapters on cell authentication, serum-free culture, apoptosis assays, FISH, genetic modification, scale-up, stem cell assays, 3-dimensional culture, tissue engineering and cytotoxicity assays. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. Everyone working in biological and medical research, whether in academia or a commercial organization, practising cell culture will benefit greatly from this book.
MoreTable of Contents:
Introduction to basic principles
Scaling-up of cell cultures
Cell line preservation and characterization
Development of serum-free media
Three dimension culture
Tissue engineering
Cytotoxicity and viability assays
Fluorescence in situ hybridization (FISH)
Genetic modification
Epithelial stem cell identification, isolation and culture
Apoptosis assays