Lipids and Membranes: Metabolism, Lipidation, and Lipid-Protein Interactions

Sorozatcím: Methods in Enzymology; 728;

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A termék adatai:

Kiadó Elsevier Science
Megjelenés dátuma 2026. január 10.

ISBN 9780443433122
Kötéstípus Keménykötés
Terjedelem258 oldal
Méret 229x152 mm
Súly 450 g
Nyelv angol

Kategóriák

Molekuláris biológia Biokémia Biofizika

Hosszú leírás:

Lipids and Membranes: Metabolism, Lipidation, and Lipid-Protein Interactions, Volume 729 provides a broad snapshot of state-of-the-art methods for studying biological lipids and biomembranes. Sections in this new release cover De novo chemoenzymatic construction of lipid membranes, Photoaffinity labeling for cholesterol metabolism, Expression and Characterization of Phospholipase Cb and Ce enzymes, Assembling Retromer-coated membrane tubules for in vitro structure-function studies, Quantitative analysis of S-acylation, Inhibition of palmitoyltransferases, Exploiting protein lipidation to create peptide-based biomaterials, and much more.

Additional sections discuss Optogenetic control of lipid-modifying enzyme activity using LOV domain insertions, Chemoproteomics approaches for inhibition of diacylglycerol kinases, Inhibition of sterol transfer protein function, Preparation of K-Ras4B containing synthetic modifications via expressed protein ligation, Regulation of lipin enzymes on membranes, Use of novel synthetic analogues to decipher the interactions of phosphatidic acids species, Analyzing lipid-nuclear receptor interactions, and much more.

Több

Tartalomjegyzék:

1. De novo chemoenzymatic construction of lipid membranes
Alessandro Fracassi
2. Photoaffinity labeling for cholesterol metabolism
Alison Ondrus
3. Expression and Characterization of Phospholipase Cb and Ce enzymes
Angeline Lyon
4. Assembling Retromer-coated membrane tubules for in vitro structure-function studies
Brett Collins
5. Quantative analysis of S-acylation
Gemma Triola
6. Inhibition of palmitoyltransferases
Hening Lin
7. Exploiting protein lipidation to create peptide-based biomaterials
James Hougland
8. Optogenetic control of lipid-modifying enzyme activity using LOV domain insertions
Jeremy Baskin
9. Chemoproteomics approaches for inhibition of diacylglycerol kinases
Ku-Lung (Ken) Hsu
10. Inhibition of sterol transfer protein function
Luca Laraia
11. Preparation of K-Ras4B containing synthetic modifications via expressed protein ligation
Mark Distefano
12. Regulation of lipin enzymes on membranes
Michael Airola
13. Use of novel synthetic analogues to decipher the interactions of phosphatidic acids species
Nicolas Vitale
14. Analyzing lipid-nuclear receptor interactions
Raymond Blind
15. Supported lipid bilayers for viral protein lipid interactions
Robert Stahelin
16. Palmitoylation-dependent probes for labeling the Golgi complex
Shinya Tsukiji
17. A real-time assay for lipidation of ATG8/LC3 using fluorerscence spectroscopy
Taki Nishimura
18. Optogenetic Phospholipase C for Measuring Phosphoinositide Turnover and DAG Generation
Toshihisa Ohtsuka
19. Targeting protein palmitoylation
Will Fuller
20. Pharmacological modulation of lipid metabolism
Wonhwa Cho