Immunology: Overview and Laboratory Manual

Immunology: Overview and Laboratory Manual

 
Kiadás sorszáma: 1st ed. 2021
Kiadó: Springer
Megjelenés dátuma:
Kötetek száma: 1 pieces, Book
 
Normál ár:

Kiadói listaár:
EUR 92.64
Becsült forint ár:
38 227 Ft (36 407 Ft + 5% áfa)
Miért becsült?
 
Az Ön ára:

35 169 (33 494 Ft + 5% áfa )
Kedvezmény(ek): 8% (kb. 3 058 Ft)
A kedvezmény csak az 'Értesítés a kedvenc témákról' hírlevelünk címzettjeinek rendeléseire érvényes.
Kattintson ide a feliratkozáshoz
 
Beszerezhetőség:

Becsült beszerzési idő: Általában 3-5 hét.
A Prosperónál jelenleg nincsen raktáron.
Nem tudnak pontosabbat?
 
  példányt

 
 
 
 
A termék adatai:

ISBN13:9783030646851
ISBN10:3030646858
Kötéstípus:Keménykötés
Terjedelem:471 oldal
Méret:235x155 mm
Súly:898 g
Nyelv:angol
Illusztrációk: 35 Illustrations, black & white; 26 Illustrations, color; 30 Tables, color
442
Témakör:
Hosszú leírás:
A two-in-one text providing teaching lab students with an overview of immunology as well as a lab manual complete with current standard exercises. 

Section I of this book provides an overview of the immune system and immunity, and includes review questions, problem sets, case studies, inquiry-based questions, and more to provide students with a strong foundation in the field. Section II consists of twenty-two lab exercises focused on key concepts in immunology, such as antibody production, cell separation, cell function, immunoassays, Th1/Th2 cytokine detection, cell and tissue culture methods, and cell and molecular biology techniques. Appendices include safety information, suggested links and readings, and standard discipline processes, protocols, and instructions.
Tartalomjegyzék:
Part I: Overview of Immunity 
1 Immunity 
2 Components of immunity 
3 Hematopoiesis
4 The Immune system: pathogen sensing and detection 
5 Lymphoid organs 
6 Innate and Adaptive immunity 
7 Leukocyte homing, migration and recirculation 
8 Apoptosis 
9 Antigens 
10 B cell development, activation and immunoglobulin structure 
11 Immunoglobulin and T cell receptor gene rearrangements
12 Major histocompatibility complex proteins 
13 T cell development and T cell receptor structure 
14 Cytokines 
15 Lymphocyte signals 
16 Complement fixation 
17 Cell mediated immunity 
18 Hypersensitivities
19 Parasite Immunity 
20 Vaccines 
21 Transplantation 
22 Immune regulation and autoimmunity 
23 Immunodeficiencies 
24 Cancer 
25 Practice questions 
26 Study guide for exam 1

Part II: Laboratory Manual
27. Immunology laboratory policies
28. Instructions for notebook entries
29. Instructions for writing lab reports 
30. Lab report topics
EXERCISE 1: Using a compound microscope
EXERCISE 2: Pipetting and dilution technique, identification of mouse lymphoid organs
EXERCISE 3: Blood Cell Preparation, Leukocyte differentiation and ABO Blood Typing
EXERCISE 4: Single Suspension of Mouse Spleen cells, Cell Viability Assays, and Identification of specific cells using surface antigens
EXERCISE 5: Isolation of mouse peritoneal macrophages: In vitro phagocytosis of bacterial cells
EXERCISE 6: Clearance of bacteria from mouse blood by the RES
EXERCISE 7: Hemolytic Plaque assay (The Jeme Plaque Assay)
EXERCISE 8: Rabbit and Mouse Immunizations- Preparation of Polyclonal antibodies and screening assays
EXERCISE 9: Determination of Antibody Titer: Screening Techniques
EXERCISE 10: Immunodiffusion and Immunoelectrophoresis
EXERCISE 11: Protein Affinity Purification of Rabbit IgG
EXERCISE 12: Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)
EXERCISE 13: Immunoblotting (Western Blotting)
EXERCISE 14: Protein Assay
EXERCISE 15: Enzyme-linked Immunosorbent Assay (ELISA)
EXERCISE 16: Enzyme-Linked Immunospot Assay (ELISPOT)
EXERCISE 17: Immunofluorescence assay (IFA) 
EXERCISE 18: Flow Cytometry
EXERCISE 19: Complement Fixation Assays
Tissue Cell Culture Techniques
EXERCISE 20: Mitogen Induced Response of Lymphocytes:
Detection of Band T cell Lymphocytes Using Immunofluorescence
EXERCISE 21: Induction of IL-2 Secretion from Mouse Lymphoma Cells
EXERCISE 22: Adoptive Transfer of Lymphocytes: Band T cell Cooperation for Antibody Production
EXERCISE 23: Macrophage Migration Inhibition
EXERCISE 24: Monoclonal Antibody Preparation
EXERCISE 25: Immunization of mice with a recombinant protein
EXERCISE 26: Polarization of T helper cells into TH1 subset
EXERCISE 27: Immunoprecipitation
EXERCISE 28: Induction of apoptosis in T lymphocytes and detection of apoptotic markers
EXERCISE 29: Cloning and sequencing of cDNAs of mouse variable regions of H and L chains of monoclonal antibodies
Appendix
Sample Practice Questions