Immune Mediators in Cancer by Vancurova, Ivana; Zhu, Yan;

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This second edition provides a comprehensive collection of classic and cutting-edge methodologies as well as bioinformatics and genome-editing approaches for cancer cells and tissues. Chapters beginning with the Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Immune Mediators in Cancer: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

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A Useful Guide for Analysis of Biomarkers in Cancer by Fluorochrome (Luminex) Technique.- Detection of Multi-analytes Using Luminex Multiplex Bead Immunoassay.- Flow Cytometry Analysis of IFNγ-induced PD-L1 Cell Surface Expression in Ovarian Cancer Cells.- Evaluating Nuclear Levels of PD-L1 in Ovarian Cancer Cells by Western Blotting.- Combined Single-Cell Measurement of Cytokine mRNA and Protein in Immune Cells.- A Beginner’s Guide to Immunohistochemistry Technique for Paraffin Sections.- Immunohistochemistry and Immunofluorescence Approaches in Cancer Research Using Paraffin-Embedded Tissue Sections.- Detection of Cytokine Receptors Using Tyramide Signal Amplification for Immunofluorescence.- Masson’s Trichrome Staining Technique to Evaluate Tissue Fibrosis.- 3D Spheroid Model: A Methodological Approach for Advancing Cancer and Immunology Research.- Analysis of IFNγ-Induced Invasion of Ovarian Cancer Cells using 3D Spheroids.- Use of Biomimetic Hydrogels and 3D Cancer Models for Biochemical and Cell Biological Assays.- Detection of Ferroptosis by Liperfluo and BODIPYTM 581/591 C11.- Methods for Measuring TNFα-induced Autophagy in Cancer Cells.- Evaluation of Metabolic Characteristics of Cancer Cells .- Assessing Intracellular Metabolism of Immune Cells in Situ in Live Zebrafish Larvae by Autofluorescence Lifetime Imaging Microscopy of NAD(P)H and FAD.- Macrophage Enhancement of Tumor Cell Extravasation and Intravasation In Vitro.- Differentiation and Polarization of THP-1 Cells into M1 and M2 Macrophages for Cancer Research.- Analysis of PD-L1 Transcriptional Activity by Chromatin Immunoprecipitation.- Semi-quantitative RT-PCR Assay for the Analysis of Alternative Splicing of Interleukin Genes.- Proximity Ligation Assay to Detect Protein-Protein Interaction in Cancer Cells.- Measuring Chimeric Antigen Receptor T Cells (CAR T Cells) Activation by Coupling Intracellular Cytokine Staining with Flow Cytometry.- Fluorescence Lifetime Imaging Application to Probe GTPase Activation in Macrophage Cell Line, using the Time-domain FastFLIM Modality.- Optimized Whole-Mount X-gal Staining to Detect Hedgehog Signaling Activity in Basal Cell Carcinoma: A Platform for Spatial Integration with Immune Analysis.- Targeting Fibrosis in Arthritis using mRNA Vaccine-Based Approach.- Engineering Mutation Clones in Mammalian Cells with CRISPR/Cas9.- Generation of Knockout Cell Lines Using CRISPR/Cas9-Based Genome Editing.- High-Throughput RNA Interference Screen Targeting Synthetic-Lethal Gain-of-Function of Oncogenic Mutant TP53 in Triple-Negative Breast Cancer.- Rapid Exploratory Data Visualization for Cancer Genomics.- Evolutionary Insights from Sequence Analysis of Interleukin 17A (IL17A).- Methods to Identify and Validate Therapeutic Targets against Cancer-Associated Fibroblasts in Head and Neck Squamous Cell Carcinoma.- Bioinformatics Analysis of Bc3, IL-8 and PD-L1 Gene Coexpression in Glioblastoma and Pan-cancer using Xena Platform.- Single-Cell 3’ mRNA Sequencing with 10x Chromium Gel Beads-in-Emulsion (GEM) Kits.- The Experimental Procedure of Somatic Hypermutation Analysis in Splenic B Cells During Primary Response.