Cerebrospinal Fluid (CSF) Proteomics
Methods and Protocols
Sorozatcím:
Methods in Molecular Biology;
2044;
Kiadás sorszáma: 1st ed. 2019
Kiadó: Humana
Megjelenés dátuma: 2020. augusztus 21.
Kötetek száma: 1 pieces, Book
Normál ár:
Kiadói listaár:
EUR 130.79
EUR 130.79
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A termék adatai:
| ISBN13: | 9781493997084 |
| ISBN10: | 1493997084 |
| Kötéstípus: | Puhakötés |
| Terjedelem: | 557 oldal |
| Méret: | 254x178 mm |
| Súly: | 1082 g |
| Nyelv: | angol |
| Illusztrációk: | 3 Illustrations, black & white; 164 Illustrations, color; 69 Tables, color |
| 689 |
Témakör:
Rövid leírás:
This volume focuses on protein analysis, including a wide range of the use of mass spectrometry and other protein methods within neurobiological disciplines. Chapters cover topics such as cerebrospinal fluid (CSF) processing and biobanking; label-free quantitative proteomics; SWATH; top-down proteomics; and experimental strategies based on other -omics applied to CSF metabolome, lipidome, and microRNAome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Cerebrospinal Fluid (CSF) Proteomics: Methods and Protocols is a valuable resource for graduate students and post-doctoral fellows interested in learning more about CSF proteotyping. It is also useful to established researchers seeking further insight into this growing field.
Hosszú leírás:
This volume focuses on protein analysis, including a wide range of the use of mass spectrometry and other protein methods within neurobiological disciplines. Chapters cover topics such as cerebrospinal fluid (CSF) processing and biobanking; label-free quantitative proteomics; SWATH; top-down proteomics; and experimental strategies based on other -omics applied to CSF metabolome, lipidome, and microRNAome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Cerebrospinal Fluid (CSF) Proteomics: Methods and Protocols is a valuable resource for graduate students and post-doctoral fellows interested in learning more about CSF proteotyping. It is also useful to established researchers seeking further insight into this growing field.
Tartalomjegyzék:
Preface?
Table of Contents?
Contributing Authors?
Part I Introduction
1. The Human Brain Proteome Project: Biological and Technological Challenges
Joaquín Fernández-Irigoyen, Fernando Corrales, and Enrique Santamaría
Part II Cerebrospinal Fluid Sample Preparation Methods for Proteomic Workflows
2. Guidelines for CSF Processing and Biobanking: Impact on the Identification and Development of Optimal CSF Protein Biomarkers
Yanaika S. Hok-a-Hin, Eline A. J. Willemse, Charlotte E. Teunissen, and Marta del Campo
3. Functional Analyses of Embryonic Cerebrospinal Fluid Proteins
Teresa Caprile, Francisco Lamus, María Isabel Alonso, Hernán Montecinos, and Angel Gato
4. CSF Sample Preparation for Data Independent Acquisition
Katalin Barkovits, Lars Tönges, and Katrin Marcus
5. Sample Fractionation Techniques for CSF Peptide Spectral Library Generation
Sandra Pacharra, Katrin Marcus, and Caroline May
Part III Shotgun Cerebrospinal Fluid Proteomics
6. Application of 2D-DIGE and iTRAQ Workflows to Analyse CSF in Gliomas
Aishwarya Rao, Kanika Mehta, Nikita Gahoi, and Sanjeeva Srivastava
7. Peptidomic Workflow Applied to Cerebrospinal Fluid Analysis
Rustam H. Ziganshin, Sergey I. Kovalchuk, and Igor V. Azarkin
8. Quantitative Evaluation of Different Protein Fractions of Cerebrospinal Fluid using 18O Labeling
Ramona Birke, Eberhard Krause, Michael Schümann, Ingolf E. Blasig, and Reiner F. Haseloff
9. A Versatile Workflow for Cerebrospinal Fluid Proteomic Analysis with Mass Spectrometry: A Matter of Choice between Deep Coverage and Sample Throughput
Charlotte Macron, Antonio Núnez Galindo, Ornella Cominetti, and Loic Dayon
10. Determination of Cerebrospinal Fluid Proteome Variations by Isobaric-Labeling Coupled to Strong Cation Exchange Chromatography and Tandem Mass-Spectrometry
Mercedes Lachén-Montes, Andrea González-Morales, Joaquín Fernández-Irigoyen, and Enrique Santamaría
11. SWATH Mass Spectrometry Applied to Cerebrospinal Fluid Differential Proteomics: Establishment of a Sample-Specific Method
Sandra I. Anjo, Cátia Santa, and Bruno Manadas
Part IV Cerebrospinal Fluid Subproteomics
12. Top-Down Proteomics Applied to Human CSF
Marina Gay, Ester Sánchez-Jiménez, Laura Villarreal, Mar Vilanova, Romain Huguet, Gianluca Arauz-Garofalo, Mireia Díaz-Lobo, Daniel López-Ferrer, and Marta Vilaseca
13. Application of an Aptamer-Based Proteomics Assay (SOMAscanTM) in Rat CSF
Alba Simats, Laura Ramiro, Joan Montaner, and Teresa García-Berrocoso
14. Monitoring the Cerebrospinal Fluid Cytokine Profile using Membrane-Based Antibody Arrays
Andrea González-Morales, Mercedes Lachén-Montes, Joaquín Fernández-Irigoyen, and Enrique Santamaría
15. Selective and Sensitive Mass Spectrometric Identification of Immune Complexe Antigens in Cerebrospinal Fluid
Nozomi Aibara and Kaname Ohyama
16. CSF N-glycoproteomics using MALDI MS Techniques in Neurodegenerative Diseases
Angela Messina, Angelo Palmigiano, Rosaria Ornella Bua, Donata Agata Romeo,
Rita Barone, Luisa Sturiale, Mario Zappia, and Domenico Garozzo
17. Deployment of Label-Free Quantitative Olfactory Proteomics to Detect Cerebrospinal Fluid Biomarker Candidates in synucleinopathies
Mercedes Lachén-Montes, Andrea González-Morales, Joaquín Fernández-Irigoyen, and
Enrique Santamaría
18. An Improved Assay for Quantitation of Cerebrospinal Fluid Cystatin C using Liquid Chromatography Tandem Mass Spectrometry
Abdullah Md Sheikh and Atsushi Nagai
19. Array-Based Profiling of Proteins and Autoantibody Repertoires in CSF
Elisa Pin, Ronald Sjöberg, Eni Andersson, Cecilia Hellström, Jennie Olofsson, August Jernbom Falk, Sofia Bergström, Julia Remnestal, David Just, Peter Nilsson, and Anna Manberg
Part V Other -Omics Applied to Cerebrospinal Fluid
20. Untargeted Metabolomics Relative Quantification by SWATH Mass Spectrometry Applied to Cerebrospinal Fluid
Vera M. Mendes, Margarida Coelho, and Bruno Manadas
21. Co-Extraction for Metabolomics and Proteomics from a Single CSF Sample
Philipp Hörmann, Katalin Barkovits, Katrin Marcus, and Karsten Hiller
22. Characterization of the Alzheimer's Disease MicroRNA Profile in Exosome Enriched CSF Samples
Javier Riancho, Ana Santurtun, and Pascual Sánchez-Juan
23. Mass-Spectrometry Applied to Human Cerebrospinal Fluid Lipidome
Laura Millán, Joaquín Fernández-Irigoyen, Enrique Santamaría, and Rebeca Mayo
Part VI Bioinformatics Applied to Cerebrospinal Fluid
24. Trait Loci Mapping and CSF Proteome
Daimei Sasayama, Kotaro Hattori, and Hiroshi Kunugi25. Essential Features and Use Cases of the Cerebrospinal Fluid Proteome Resource (CSF-PR)
Astrid Guldbrandsen, Yehia Farag, Ragnhild Reehorst Lereim, Frode S. Berven, and Harald Barsnes
26. Bioinformatics to Tackle the Biological Meaning of Human Cerebrospinal Fluid Proteome
Fábio Trindade, Rita Nogueira-Ferreira, Paulo Bastos, Francisco Amado, Rita Ferreira, and Rui Vitorino 
