The Optical Clearing Method
A New Tool for Clinical Practice and Biomedical Engineering
Series: SpringerBriefs in Physics;
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Product details:
- Edition number 1st ed. 2019
- Publisher Springer International Publishing
- Date of Publication 6 December 2019
- Number of Volumes 1 pieces, Book
- ISBN 9783030330545
- Binding Paperback
- No. of pages177 pages
- Size 235x155 mm
- Weight 454 g
- Language English
- Illustrations XI, 177 p. 53 illus., 47 illus. in color. Illustrations, black & white 18
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Long description:
This book describes the Optical Immersion Clearing method and its application to acquire information with importance for clinical practice and various fields of biomedical engineering. The method has proved to be a reliable means of increasing tissue transparency, allowing the investigator or surgeon to reach deeper tissue layers for improved imaging and laser surgery. This result is obtained by partial replacement of tissue water with an active optical clearing agent (OCA) that has a higher refractive index and is a better match for the refractive index of other tissue components. Natural tissue scattering is thereby reduced. An exponential increase in research using this method has occurred in recent years, and new applications have emerged, both in clinical practice and in some areas of biomedical engineering. Recent research has revealed that treating ex vivo tissues with solutions containing active OCAs in different concentrations produces experimental data to characterize drug delivery or to discriminate between normal and pathological tissues. The obtained drug diffusion properties are of interest for the pharmaceutical and organ preservation industry. Similar data can be estimated with particular interest for food preservation. The free water content evaluation is also of great interest since it facilitates the characterization of tissues to discriminate pathologies. An interesting new application that is presented in the book regards the creation of two optical windows in the ultraviolet spectral range through the application of the immersion method. These induced transparency windows open the possibility to diagnose and treat pathologies with ultraviolet light. This book presents photographs from the tissues we have studied and figures that represent the experimental setups used. Graphs and tables are also included to show the numerical results obtained in the sequential calculations performed.
MoreTable of Contents:
Chapter1. Tissue Optics.- Chapter2. Controlling the optical properties of biological materials.- Chapter3. Typical optical clearing agents.- Chapter4. Major optical clearing mechanisms.- Chapter5. Measurements during optical clearing.- Chapter6. Data that can be acquired from optical clearing studies.- Chapter7. Optical clearing and tissue imaging.- Chapter8. Other applications of OCAs.- Chapter9. Future perspectives of the optical clearing method.
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