Stem Cell Laboratory Techniques
A Guide for Researchers and Students
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Product details:
- Publisher Elsevier Science
- Date of Publication 31 May 2023
- ISBN 9780128237298
- Binding Paperback
- No. of pages240 pages
- Size 234x190 mm
- Weight 500 g
- Language English
- Illustrations 70 illustrations (20 in full color) 466
Categories
Long description:
Stem Cell Laboratory Techniques: A Guide for Researchers and Students introduces the reader to stem cell culture, handling techniques and versatile applications used by researchers. Sections introduce stem cells, including definitions, types and basic use of stem cells in biomedical science research. The book explains laboratory procedures and techniques ranging from the extraction of stem cells from animals, cell seeding and culture, harvest and maintenance of stem cells, stem cell characterization, accurate recording, quality control, and more. In addition, it guides researchers on topics such as transcriptome analysis, proliferation study analysis, and microphysiological study.
Final sections cover useful and recent applications in stem cells, such as gene editing techniques and the preparation of stem cells for in vitro study, as well as stem cell lab design and equipment used in the lab. Lastly, human and animal research ethics are discussed.
MoreTable of Contents:
1. Introduction: Stem Cell Definition, Types of Stem Cells, Use and Application of Stem Cell
2. Extraction of Stem Cells from Animal Models
3. Harvest and Culture of Stem Cells
4. Establishment of Amniotic fluid Stem Cell
5. Stem Cell Characterization
6. Good Recording and Quality Control
7. Differentiation Techniques for Confirmation Studies
8. Transcriptomic Analysis
9. Proliferation Study and Analysis
10. Single Cell Isolation Methods and their Impact on Stem Cell Study
11. Gene Editing Techniques for Stem Cell
12. Preparation of Cells for in vitro study
13. Stem Cell Lab Design and Equipment
14. Human and Animal Research Ethics
15. Organoids
