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46 819 Ft
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Product details:
- Publisher OUP Oxford
- Date of Publication 29 November 2001
- ISBN 9780199638826
- Binding Paperback
- No. of pages312 pages
- Size 246x190x19 mm
- Weight 696 g
- Language English
- Illustrations numerous figures 0
Categories
Short description:
This is a comprehesive review of new methods that can be used for studying cellular receptors and examines how each technique can be used on different receptor types.
MoreLong description:
Given the number of exciting developments across the whole spectrum of receptor research in recent years, the editors have not restricted themselves to one particular approach or class of receptors. Thus the studies within range from G protein-coupled surface receptors, to the delivery of antisense DNA inside living cell systems. A distinguished team of contributors cover these diverse areas, identifying any difficulties likely to be encountered and appropriate steps
to overcome them.
Wherever appropriate, the theoretical basis of each topic is explained first so that the results emerging from the practical procedures can be fully understood. Anyone with an interest in receptor structure and function will find this book an invaluable resource.
Table of Contents:
Probing G protein-coupled receptors by fluorescence spectroscopy
From receptor to endogenous ligand
Point mutations and chimeric receptors in studies of ligand binding domains
Determination of the subunit composition of native and cloned NMDA receptors
Evaluating receptor stoichiometry by fluorescence resonance energy transfer (FRET)
Monitoring intracellular trafficking of receptors
Monitoring the turnover kinetics of G protein-coupled receptors
Real time receptor function in vitro: microphysiometry and fluorometric imaging plate reader (FLIPR)
Regulation of receptors and receptor-coupled ion channels by protein phosphorylation
Using confocal imaging to measure changes in intracellular ions
Monitoring nuclear receptor function
In vivo gene transfer of antisense to receptors
