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Product details:
- Publisher IRL Press
- Date of Publication 14 March 1996
- ISBN 9780199634842
- Binding Paperback
- No. of pages268 pages
- Size 235x156x16 mm
- Weight 471 g
- Language English
- Illustrations halftones, line figures, tables 0
Categories
Short description:
This book brings together methods for preparing and maintaining cell cultures from a diverse range of neural tissues and cells, from both the central and peripheral nervous systems.
MoreLong description:
The complexity and cellular heterogeneity of the vertebrate nervous system makes it difficult to study neural processes at the cellular and molecular levels. The establishment of cell culture model systems for almost every region and cell type in the nervous system has therefore been crucial in facilitating such studies and in heralding some key breakthroughs in our understanding of the underlying mechanisms.
Neural Cell Culture: A Practical Approach brings together methods for preparing cultures from a diverse range of neural tissues and cells, from both the central and peripheral nervous systems of mammalian and avain species. Important features of this volume are the clearly illustrated dissection guides accompanying each chapter, methods for enriching and purifying distinct cell types, and guidance on optimizing cell viability and growth. Each chapter includes valuable step-by-step protocols, hints, and tips for success, advice, and background information.
Sixteen chapters provide detailed methodological protocols on how to culture different types of neural tissue ... These chapters have been written by experts who have extensive experience with the methods in their own laboratories; hence, they typically reflect the product of years of experience with variants of the protocols described. The book is very accessible and easy to use. It is well organized, and indexed ... Nothing teaches like experience, and at least this book will give you some ideas about the experience of others that will help to guide your progress. For the neuroscientist engaged in neural cell culture this manual may be useful as a general reference, and for anyone who has immediate need for a particular chapter, this manual could indeed prove a very helpful resource.
Table of Contents:
Part I - CNS Neurons
Cerebellum: granule cells
Cerebellar Purkinje neurons
Nigral & straital neurons
Septal neurons
Proliferation & differentiation in retinal cell cultures
Purified embryonic motoneurones
Part II - CNS glia and non-neuronal cells
Astrocytes
Oligodendrocyte lineage cells from neonatal rat brain
Micrglia: the tissue macrophage of the CNS
Brain endothelium
Part III - PNS neurons and glia
Neural crest cells: strategies to generate lineage diversifaction in vitro
Avian cilliary and lumbar sympathetic ganglion neurons
Enteric ganglion cells
Mouse olfactory epithelium
Schwann cell culture
