Multimedia Methods in Molecular Biology for Windows by Sheardown, Steve; Dixon, Mark; Partridge, T.; Rickwood, D.;

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Out of print

Product details:

Publisher Springer Verlag
Date of Publication 31 March 1997
Number of Volumes 1 pieces Compact disk (CD-ROM)

ISBN 9780412730702
Binding CD-ROM
No. of pages pages
Size 0x0 mm
Language English

Long description:

The first title in an important new series, this revolutionary new reference source and training aid provides information on over 200 key molecular biology protocols. Each method contains detailed step-by-step instructions to show procedures and results clearly, using video clips with sound commentary, illustrations, and animations to illustrate techniques. A glossary, hazard information and hints and tips on the methods are also included. Links are provided between related methods and in addition, a unique protocol building feature enables users to link-up protocols, to include their own preferred procedures. Designed for research laboratories, it is expected that it will be used principally for training new staff and as a reference tool and laboratory documentation database.

Affinity chromatography; agarose digenstion; agarose gel electrophoresis; bacterial alkaline lysis; Bacterial transformation; Band stab procedure; Blotting gels; blunt ending DNA fragments; buoyant density centrifugation; capillary blotting; CHEF electrophoresis; chloroplast isolation; concentrating nucleic acid samples; Cosmid fingerprinting; Cosmid library; denaturing agarose gel electrophoresis; denaturing polyacryamide gel electrophoresis; density gradient centrifugation; dephosphorylation of DNA; differential centrifugation; differential display; DNA ligation in gel slices; DNA sequencing; DNA: protein interactions; Electroblotting; Electroporation of bacteria; Electroporation of mammilian cells; Elution of DNA from gels; Exon trapping; Exonuclease III; Gel electrophoresis; Gel shift/retardation assay; genomic DNA isolation; Heteroduplex analysis; homgenization; homogenization; Immunodetection; Immunoelectron labeling; Immunofluorescence; Immunolabeling; Immunological screening of cDNA expression libraries; Immunoprecipitation; In situ hybridization; in vitro transcription; isoelectric focusing; Isolation of DNA from gel segments; isopycnic density gradient centrifugation; library screening; Linkers; Magnetic beads; Manual Cycle Sequencing; Methylation analysis; mitochondrial isolation; modification interference; molecular weight markers; mtDNA isolation; non denaturing gel electrophoresis; non denaturing mutation-detection-enhancement (MDE) polyacrylamide gels; northern blotting; nuclear run-on assays; nuclei isolation; nucleoli isolation; P1 phage; Partial digestion of genomic DNA; PCR; Page display libraries; plant protoplast; plasmid DNA isolation; Polyacrylamide gel electrophoresis; Polymerase chain reaction (PCR); polysome isolation; Primer extension; pulsed field gel electrophoresis; radiolabeling DNA; Reporter genes; Restriction digestion; Restriction Mapping of cloned DNA; Reverse transcription; Ribonulcease H digestion of RNA; ribosome isolation; RNA; RNA mapping; RNA protein interactions; RNA sequencing; RNA splicing assays; SDS-PAGE; Single-stranded Conformational Polymorphism (SSCP) Analysis; Site directed Mutagenesis; slot blots; subcloning; terminal transferase; tobacco acid phosphatase; total RNA isolation; velocity gradient centrifugation; western blotting; yeast spheroplast.

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