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Product details:
- Publisher OUP Oxford
- Date of Publication 4 May 2000
- ISBN 9780199637225
- Binding Paperback
- No. of pages510 pages
- Size 246x189x28 mm
- Weight 1136 g
- Language English
- Illustrations 4 pp colour plates, numerous halftones and line figures 0
Categories
Short description:
Monoclonal Antibodies: A Practical Approach deals with the preparation, testing, derivation, and applications of monoclonal antibodies. It will be an invaluable laboratory bench companion to both new and experienced immunologists.
MoreLong description:
Monoclonal Antibodies: A Practical Approach covers the preparation, testing, derivation, and applications of monoclonal antibodies. New immunological techniques incorporating tried and tested methodologies are described, making the book of interest to established and inexperienced immunologists. Both the standard somatic hybridization technique and recombinant techniques, including the use of phage libraries, for the preparation of rodent and human
monoclonal antibodies are described. Protocols for both the small and large scale production are detailed, as well as purification and labelling (with both radioisotopes and non-radioisotopes) methods. The applications of monoclonal antibodies in immunoblotting, enzyme linked immunoassays, immunofluorescence, and
FACS analysis are all covered in detail. Finally protocols are given for the use of monoclonal antibodies in rheumatoid arthritis, tissue typing, detecting DNA modified during chemotherapy, and in the clinical analysis of transplantation samples for malignancy. This book will therefore be an invaluable laboratory companion to anyone using monoclonal antibodies in their research.
A useful and practical addition to the field of cell biology ... many research scientists, students and technicians will benefit from step-by-step experimental protocols demonstrating the power of hybridoma technologies.
Table of Contents:
Preparation of Rodent Monoclonal Antibodies by in vitro Somatic Hybridization
Preparation of Recombinant Antibodies From Immune Rodent Spleens and the Design of Their Humanisation by CDR Grafting
Selection of Antibodies From Phage Libraries of Immunoglobulin Genes
Arm Complexes for in vitro Display and Evolution of Antibody Combining Sites
Human Monoclonal Antibodies to Blood Group Antigens
Laboratory Based Methods for Small-Scale Productions of Monoclonal Antibodies
Isolation and Purification of Antibodies From Tissue Culture Supernatant
Antibody Production in Plants
Radiolabelling of Monoclonal Antibodies
Non-Radioactive Antibody Probes
Immunogold Probes for Light and Electron Microscopy
Characterization of Cellular Antigens Using Monoclonal Antibodies
Immunoassays
Immunoaffinity Chromatography of Macromolecules
Immunochemical Detection of BrdUrd-Labelled Nuclei for Monitoring Cell Kinetics
Immunofluorescence Microscopy
Analysis of Clinical Haematological Samples in Transplantation for Cancer
Immunocytochemical Staining of Cells and Tissues for Diagnostic Applications
Detection of Chemically Modified DNA in Lymphocytes of Patients Undergoing Chemotheraphy
Monoclonal Antibody Therapy in Organ Transplantation
Monoclonal Antibody Therapy in Rheumatoid Arthritis
