Mitosis
EUR 128.39
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| ISBN13: | 9781071619063 |
| ISBN10: | 1071619063 |
| Binding: | Paperback |
| No. of pages: | 256 pages |
| Size: | 254x178 mm |
| Weight: | 515 g |
| Language: | English |
| Illustrations: | 11 Illustrations, black & white; 44 Illustrations, color; 44 Tables, color |
| 0 |
This detailed volume collects a selection of key techniques for studying cell division, representing multiple model systems and varied scales of approach. Over the past 20 years, a series of revolutions in experimental molecular biology, including chimeric fluorescent protein expression, multiple advanced modes of quantitative microscopy, and array of small molecule inhibitors, proteomic profiling, and gene silencing/manipulation/analysis, has advanced the mitosis field to a point where single cell biology not only allows for imaging/localization studies, but also for quantitative analysis and sequencing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Mitosis: Methods and Protocols provides a repository of techniques and approaches for those working in the field as well as a working resource for those venturing into the study of mitosis for the first time.
1. Use of Echinoderm Gametes and Early Embryos for Studying Meiosis and Mitosis
Debadrita Pal, Florencia Visconti, Silvia P. Sepúlveda
-Ramírez, S. Zachary Swartz, and Charles B. Shuster
2. Methods for Investigating Cell Division Mechanisms in C. elegans
Ian D. Wolff, Nikita S. Divekar, and Sarah M. Wignall
3. Visualizing the Dynamics of Cell Division by Live Imaging Drosophila Larval Brain Squashes
Brandt Warecki, Ian Bast, and William Sullivan
4. Imaging Chambers for Arabidopsis Seedlings for Mitotic Studies
Sidney L. Shaw, Mathew Siebe, and Timothy Cioffi
5. Live Fluorescence Imaging of Chromosome Segregation in Cultured Cells
John R. Daum, Casey O. DuBose, Sushama Sivakumar, and Gary J. Gorbsky
6. Staining of the Actin Cytoskeleton during Cell Division in Budding Yeast and Mammalian Cells
Katie B. Shannon
7. Synchronizing Mammalian Cells for Mitotic Analysis of the Localization of Survivin
Sally P. Wheatley
8. Using Microinjection of Mammalian Cultured Cells to Study Cell Division
Charles Day, Alyssa Langfald, and Edward H. Hinchcliffe
9. In Vitro and In Vivo Approaches to Study Kinetochore
-Microtubule Attachments during Mitosis
Sana Afreen, Amit Rahi, Adriana G. Landeros, Manas Chakraborty, Richard J. McKenney, and Dileep Varma
10. Quantifying Changes in Chromosome Position to Assess Chromokinesin Activity
Alex F. Thompson, Sarah Vandal, and Jason Stumpff
11. Chemical Biology of Mitotic Spindle Assembly Motors
April Solon and Ryoma Ohi
12. Deploying Kinase Inhibitors to Study Pediatric Gliomas
Benjamin T. Himes, Liang Zhang, and David J. Daniels
13. Identifying Cyclin A/Cdk1 Substrates in Mitosis in Human Cells
Ana Maria G. Dumitru and Duane A. Compton
14. Degron Tagging Using mAID and SMASh Tags in RPE
-1 Cells
Adrijana Crncec and Helfrid Hochegger
15. Centromere Tension Measurement in Budding Yeast Mitosis
Soumya Mukherjee and Melissa K. Gardner
16. Simulating Dynamic Chromosome Compaction: Methods for Bridging In Silico to In Vivo
Yunyan He, David Adalsteinsson, Benjamin Walker, Josh Lawrimore, M. Gregory Forest, and Kerry Bloom
17. Using FLIM
-FRET for Characterizing Spatial Interactions in the Spindle
Stephanie C. Ems
-McClung and Claire E. Walczak
18. Imaging Mitosis with Lattice LightSheet
Andrew Barazia and Colin Monks
