MicroRNA Profiling by Rani, Sweta;

Methods and Protocols

Series: Methods in Molecular Biology; 1509;

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Product details:

Edition number Softcover reprint of the original 1st ed. 2017
Publisher Springer New York
Date of Publication 20 June 2018
Number of Volumes 1 pieces, Previously published in hardcover

ISBN 9781493982257
Binding Paperback
See also 9781493965229
No. of pages246 pages
Size 254x178 mm
Weight 4946 g
Language English
Illustrations XI, 246 p. 41 illus., 32 illus. in color. Illustrations, black & white

Long description:

This volume includes comprehensive descriptions of miRNA biogenesis and their role in the development and progression of various human diseases. The first few chapters of MicroRNA Profiling: Methods and Protocols discuss the effects of over-expressing and repressing of a target miRNA and their effects on cell viability and proliferation. The next few chapters explore the protocols for total RNA isolation from cells and cell-derived product including formalin fixed paraffin embedded tissue and plant tissue. The last few chapters discuss isolation and characterization of exosomes from medium conditioned by cell lines, serum, and plasma specimens. This book also includes discussions of several software tools, such as miRandola, PicTar, DIANA, and miRWalk. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, MicroRNA Profiling: Methods and Protocols is a valuable resource for anyone interested in the field of Micro RNAs.

MiRNA Biogenesis and Regulation of Diseases: An Overview.- Assessment of Basic Biological Functions Exerted by miRNAs.- Extraction of miRNAs from Formalin Fixed Paraffin Embedded (FFPE) Tissues.- MiRNA Isolation from Plants Rich in Polysaccharides and Polyphenols.- MicroRNA Profiling of Exosomes.- MiRNA Profiling in Human Induced Pluripotent Stem Cells.- MiRNA Expression in Cystic Fibrosis Bronchial Epithelial Cells.- TaqMan Low Density Array: MicroRNA Profiling for Biomarker and Oncosuppressor Discovery.- Detection of MicroRNAs in Brain Slices Using In Situ Hybridization.- Exosomal MicroRNA Discovery in Age-Related Macular Degeneration.- Profiling the MicroRNA Payload of Exosomes Derived from Ex Vivo Primary Colorectal Fibroblasts.- Circulating MicroRNAs in Cancer.- Profiling Circulating MiRNAs from the Plasma of Individuals with Metabolic Syndrome.- Manipulating MiRNA Expression to Uncover Hidden Functions.- Analysis of the Distribution Profiles of Circulating MicroRNAs by Asymmetrical Flow Field Flow Fractionation.- MicroRNA Expression Profiling Using Agilent One-Color Microarray.- A Multiplex Ligation Assay for MiRNA Copy Number Profiling.- Practical Bioinformatics Analysis of MiRNA Data Using Online Tools.- Visualization and Analysis of MiRNA-Targets Interactions Networks.- Guidelines on Designing MicroRNA Sponges: From Construction to Stable Cell Line.- Customization of Artificial MicroRNA Design.

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