Laboratory Manual of Reproductive Biology and Assisted Reproductive Technologies by Taneja, Jyoti; Bhardwaj, Priya;

Short description:

The manual Reproductive Biology, Endocrinology and Assisted Reproductive Technology serve as a comprehensive, step-by-step guide for undergraduate and postgraduate students of Zoology, Life Sciences, Biotechnology, and Medical Sciences. 

Long description:

The Laboratory Manual of Reproductive Endocrinology and Assisted Reproductive Technology serves as a comprehensive, step-by-step guide for undergraduate and postgraduate students of zoology, life sciences, biotechnology and the medical sciences. It integrates fundamental concepts with advanced experimental approaches, beginning with animal handling and histological examinations and progressing to modern techniques such as hormonal assays, protein profiling, oocyte retrieval, embryo grading and infertility investigations.

Detailed coverage of assisted reproductive technologies (ART) including in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI), cryopreservation and embryo transfer is complemented by discussions on ethics, frameworks and the role of artificial intelligence (AI) in reproductive health. To support effective learning, the manual incorporates illustrations, labeled images, video guide, a glossary and references, making it an invaluable resource for both classroom teaching and online learning. Scientifically rigorous and practically oriented, it equips learners with conceptual clarity, technical skills and ethical insight into the evolving field of reproductive biology.

Key Features:

• Step-by-step coverage of both basic and advanced experimental procedures in reproductive biology and endocrinology


• Detailed modules on ART, including IVF, ICSI, embryo grading and cryopreservation


• Integration of contemporary issues such as ethics and AI in reproductive health


• Rich visual aids, including labeled images, illustrations, histological sections and a step-by-step surgical video guide, to support effective learning


• Glossary of key terms and references to relevant literature for further study

Table of Contents:

Chapter 1: Set up and maintenance of Rodent Animal House facility; Chapter 2: Display of major endocrine glands and reproductive organ system in male and female rats; Chapter 3: Microscopic examinations of histological sections using illustrations/permanent slides/photomicrographs of human/rat; Chapter 4: Surgical techniques: Principles of surgery in endocrinology; Chapter 5: Dissect and display of the hypothalamic-hypophyseal organ system in rats; Chapter 6: To study the estrous cycle by observing the vaginal smear of adult female rats (Sprague-Dawley) under normal conditions; Chapter 7: Study of Exfoliative cytology of human cervix and vagina through micrographs; Chapter 8: Study of Sperm counting, Sperm Vitality and Sperm Motility in rats; Chapter 9: Study the Effect of Cryptorchidism on Sperm Count and Motility in Rats; Chapter 10: Study of Protein Profile of Rat Ovarian Extract by SDS-PAGE; Chapter 11: Separation of steroid hormones by Thin layer Chromatography and Paper chromatography; Chapter 12: To determine and quantify glycogen/ascorbic acid levels in rat uterine tissue; Chapter 13: To determine and quantify fructose in the dorsolateral prostate and coagulating glands of rat; Chapter 14: To Estimate Total Estrogens in Human Serum Samples Using Competitive ELISA technique; Chapter 15: To Understand the Methodology of a Pregnancy Test Kit; Chapter 16: Birth control methods: Study of modern contraceptive devices; Chapter 17: Retrieval of oocytes by induced superovulation in immature mice; Chapter 18: Study of causes of infertility in males and females and infertility investigation using secondary data, models and pictographs; Chapter 19: Setting up and Study of IVF Laboratory Culture Conditions; Chapter 20: Oocyte scoring and embryo quality grading in IVF for successful implantation; Chapter 21: Study of Assisted Reproductive Techniques; Chapter 22: Assisted Reproductive Technology: Ethical principles and laws in India; Chapter 23: Artificial Intelligence tools for Detection and Management of Sexually Transmitted Infections and Women's Reproductive Health: A Practical Approach for Undergraduate Students.