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Product details:
- Publisher IRL Press
- Date of Publication 22 August 1996
- ISBN 9780199635597
- Binding Paperback
- No. of pages334 pages
- Size 235x156x18 mm
- Weight 580 g
- Language English
- Illustrations halftone and line figures, tables 0
Categories
Short description:
Free radicals - reactive, short-lived molecules - are now known to play an important role in a large number of human diseases, including heart disease and cancer, and their study in biological systems has increased enormously in recent years. This volume brings together practical techniques for detecting and measuring free radicals, and for studying their effects. It will be of interest to a wide cross-section of biochemists and clinicians.
MoreLong description:
Free radicals - reactive, short-lived molecules - are now known to play an important role in a large number of human diseases, including heart disease and cancer, and their study in biological systems has increased enormously in recent years. This volume brings together practical techniques for detecting and measuring free radicals, and for studying their effects. It will be of interest to a wide cross-section of biochemists and clinicians.
will greatly assist any laboratory wishing to enter the fray in what must be one of the most exciting areas of medicine, nutritional science and food technology ... the introductory sections to each chapter are in themselves small masterpieces of explanation of the states of the arts for which the protocols are relevant. Thus this book will be not just a laboratory manual but should be a bestseller in the student market.
Table of Contents:
Introduction
Direct and indirect measurements of free radicals by physico-chemical methods
In vitro and in vivo detection of free radicals metabolites with electron spin resonance
The application of high-field nuclear magnetic resonance spectroscopy to the detection of products arising from the reactions of free radicals in chemical model systems, biosystems, and foodstuffs
Direct and indirect measurement of free radicals by other methods
Intrinsic (low-level) chemiluminescence
Visual assessment of oxidative stress by multifunctional digital microfluorography
Salicylic acid and phenylalanine as probes to detect hydroxyl radicals
Measurement of free radical products
Lipids (i): peroxides and other products
Lipids (ii): quantitative analysis of 4-hydroxyl 2-nonenal
Lipids (iii): F2-isoprostanes: prostaglandin-like products of lipid peroxidation
Proteins (i): determination of carbonyl groups in oxidized proteins
Proteins (ii): protein hydroperoxides, protein hydroxides, and protein-bound DOPA
Carbohydrates: investigating the effects of oxygen free radicals and carbohydrates in biological systems
Nucleic acids: measurement of products of free radical attack on nucleic acids
Measurement of antioxidants
Glutathione
Glutathione peroxidase: activity and steady-state level of mRBA
Superoxide dismutase
Application of deuterium labelling and gas chromatography-mass spectrometry to the measurement of vitamin E bioavailability in tissue and body fluids
Selective and sensitive measurement of vitamin C, ubiquinol-10, and other low molecular weight antioxidants
Measurement of antioxidant gene expression
Appendix
