FISH (Fluorescence In Situ Hybridization)
Series: Practical Approach Series; 260;
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Product details:
- Publisher OUP Oxford
- Date of Publication 4 July 2002
- ISBN 9780199638840
- Binding Paperback
- No. of pages274 pages
- Size 246x190x17 mm
- Weight 671 g
- Language English
- Illustrations numerous figures, halftones and tables; 16pp colour plates 0
Categories
Short description:
FISH (Fluorescence In Situ Hybridization) is a highly versatile technique that is constantly being applied to new branches of research, particularly as more and more probes in regions of the genome associated with genetic diseases or cancer become available through the Human Genome Project. With over 100 easy to follow protocols, this book will be of significant interest to students and technicians in both clinical service and basic science laboratories who employ
genetic or genomic technologies.
Long description:
This convenient laboratory manual covers the theory and application of all the commonly used FISH procedures for both the research and clinical service laboratory. Readers are provided with the theoretical principles which underlie each procedure, and are then guided through the protocols in a systematic, easy to follow manner. Because FISH procedures are particularly sensitive to background and subtle variations in sample quality, the trouble-shooting sections are
invaluable to the beginner interested in developing FISH capabilities in their laboratory. Areas covered include probe production and labelling; FISH gene mapping; murine, fiber, and RNA FISH; CGH and microchips; 3D FISH; SKY and M-FISH; FISH and microarrays; and FISH in clinical cytogenetics. The
information is easy to follow and includes a large number of useful reference web sites in addition to a comprehensive and up to date bibliography providing background to all the advanced molecular cytogenetics The information is easy to follow and includes a large number of useful reference web sites in addition to a comprehensive and up to date bibliography providing background to all the advanced molecular cytogenetics techniques in current use. This book will be of particular value to
scientists, technologists, and students in the areas of molecular pathology, cancer biology, genomics, haematology, oncology, clinical cytogenetics, molecular diagnostics and genetics.
As a resource for both the researcher and the clinician, this book should serve admirably. It should be a welcome addition to any technical library.
Table of Contents:
Introduction
Labelling Methods
FISH Mapping
Murine FISH
Fiber FISH
RNA FISH
Three Dimensional FISH
CGH
Clinical Cytogenetics
Multicolour FISH and SKY
cDNA Microarrays and FISH
