Enzymology at the Membrane Interface: Intramembrane Proteases
Series: Methods in Enzymology; 584;
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Product details:
- Publisher Elsevier Science
- Date of Publication 5 January 2017
- ISBN 9780128122136
- Binding Hardback
- No. of pages494 pages
- Size 228x152 mm
- Weight 930 g
- Language English 0
Categories
Long description:
Enzymology at the Membrane Interface: Intramembrane Proteases, Volume 584, the latest release in the Methods in Enzymology series, covers a subset of enzymes that work in the environment of the biological cell membrane. This field, called interfacial enzymology, involves a special series of experimental approaches for the isolation and study of these enzymes.
MoreTable of Contents:
1. Biochemical Characterization of Function and Structure of RseP, an Escherichia Coli S2P Protease
Y. Hizukuri, K. Akiyama and Y. Akiyama
2. Signal Peptidase Enzymology and Substrate Specificity Profiling
R.E. Dalbey, D. Pei and ï¿1⁄2.D. Ekici
3. Screening and Characterization Strategies for Nanobodies Targeting Membrane Proteins
S. Veugelen, M. Dewilde, B.D. Strooper and L. Chï¿1⁄2vez-Gutiï¿1⁄2rrez
4. Probing the Activity of Eukaryotic Rhomboid Proteases in Vitro
B. Cordier and M.K. Lemberg
5. Expression, Purification, and Enzymatic Characterization of Intramembrane Proteases
R. Zhou, Y. Shi and G. Yang
6. Analyzing Amyloid-? Peptide Modulation Profiles and Binding Sites of ?-Secretase Modulators
J. Trambauer, A. Fukumori, B. Kretner and H. Steiner
7. Probing the Structure and Function Relationships of Presenilin by Substituted-Cysteine Accessibility Method
T. Tomita
8. A New Method to Determine the Transmembrane Conformation of Substrates in Intramembrane Proteolysis by Deep-UV Resonance Raman Spectroscopy
J.W. Cooley, A. Abdine, M. Brown, J. Chavez, B. Lada, R.D. JiJi and I. Ubarretxena-Belandia
9. An Inducible Reconstitution System for the Real-Time Kinetic Analysis of Protease Activity and Inhibition Inside the Membrane
R.P. Baker and S. Urban
10. Production of Recombinant Rhomboid Proteases
E. Arutyunova, R. Panigrahi, K. Strisovsky and M.J. Lemieux
11. Mechanism and Inhibition of Rhomboid Proteases
K. Strisovsky
12. Enzymatic Assays for Studying Intramembrane Proteolysis
D.M. Bolduc, D.J. Selkoe and M.S. Wolfe
13. Methods for Sï¿1⁄2tructural and Functional Analyses of Intramembrane Prenyltransferases in the UbiA Super Family
Y. Yang, N. Ke, S. Liu and W. Li
14. Functional Study of the Vitamin K Cycle Enzymes in Live Cells
J.-K. Tie and D.W. Stafford
15. Activity Assays for Rhomboid Proteases
E. Arutyunova, K. Strisovsky and J. Lemieux
