Cytoskeleton: Signalling and Cell Regulation
A Practical Approach
Series: Practical Approach Series; 221;
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Product details:
- Publisher Oxford University Press
- Date of Publication 16 December 1999
- Number of Volumes laminated boards
- ISBN 9780199637829
- Binding Hardback
- No. of pages308 pages
- Size 241x161x22 mm
- Weight 674 g
- Language English
- Illustrations 2 colour illustrations, numerous halftones, tables 0
Categories
Short description:
This book provides detailed descriptions of experimental methods that bridge active and fertile areas of cell biological research. It is particularly focused on methods for understanding dynamic changes in cell structure and their relationship to the regulation of cell behaviour.
MoreLong description:
This book provides descriptions of experimental methods in research on the cytoskeleton and its relationships to signaling and cell regulation. Thus, it bridges two active and fertile areas of research. The focus is directed particularly towards methods which take advantage of recent advances in molecular biology, microscopy and immunological assays. A second emphasis is on methods for understanding dynamic changes in cells. A third emphasis is on the formation
and turnover of macromolecular and supramolecular complexes, which are so important in driving cell regulation and the behaviour of cytoskeletal elements. A combination of practical advice and detailed protocols should make this book valuable for both novice and experienced workers in these
burgeoning fields.
Table of Contents:
Preface
Introduction: The cytoskeleton in the transduction of signal and regulation of cellular function
Assaying binding and covalent modifications of cytoskeletal proteins
Methods to study actin assembly and dynamics in yeast
Signaling complexes: Association of signaling proteins with the cytoskeleton
Ras-related GTPases and the cytoskeleton
Applications for analysis of contractile functions in dividing and migrating tissue culture cells
Video microscopy: Protocols for examining the actin-based motility of Listeria, Shigella, Vaccinia, and Lanthanum-induced endosomes.
Structural studies on tubulin and microtubules
Regulation of Intermediate Filament Dynamics
The study of mRNA-cytoskeleton interactions and mRNA sorting in mammalian cells
Cell shape control and mechanical signaling through the cytoskeleton
Appendix
Index
