Current Methods in Muscle Physiology
Advantages, Problems and Limitations
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Product details:
- Publisher OUP Oxford
- Date of Publication 22 January 1998
- ISBN 9780198523970
- Binding Hardback
- No. of pages384 pages
- Size 242x161x25 mm
- Weight 787 g
- Language English
- Illustrations halftones, line figures, tables 0
Categories
Short description:
Despite extensive physiological, biochemical, and structural studies, the mechanisms of muscle contraction operating in living muscle fibres are still not well understood. This book aims to describe critically various experimental methods currently used in the field of muscle research. For each method discussed, there is a comprehensive description of its advantages, problems, and limitations. Each chapter also contains a summary of the central results to have been obtained using each method. Comprehensively written by experts in their respective fields, this book will be of interest to all investigators with an interest in muscle physiology.
MoreLong description:
Despite extensive physiological, biochemical, and structural studies, the mechanisms of muscle contraction operating in living muscle fibres are still not clearly understood. This book aims to describe and assess various experimental methods currently used in the field of muscle research. For each method discussed, there is a comprehensive description of its advantages, problems, and limitations. Each chapter also contains a summary of the central results to have been obtained using each method. Comprehensively written by experts in their respective fields, this book will be of interest to all investigators in muscle physiology.
MoreTable of Contents:
Chapter 1 - Combined biochemical and mechanical experiments
Muscle mechanics I - intact single muscle fibres
Muscle mechanics II - skinned muscle fibres
The use of caged compounds
The use of fluorescent nucleotides
The use of peptide mimetics
The use of recombinant molecules
In vitro motility assay
Chapter 2 - Combined structural and mechanical experiments
The use of fluorescent probes
The use of spin probes
Time resolved X-ray diffraction
Electron microscopy coupled with quick freezing
Chapter 3 - The use of Ca2+ indicators
Chapter 4 - Electrophysiological techniques
Chapter 5 - Techniques for muscle energetics
