Combinatorial Biotechnology
Methods for Synthesis of Peptide, Oligonucleotide and Oligosaccharide Libraries and Applications
Series: Progress in Biochemistry and Biotechnology;
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Product details:
- Publisher Elsevier Science
- Date of Publication 1 June 2026
- ISBN 9780443275609
- Binding Paperback
- No. of pages325 pages
- Size 235x191 mm
- Weight 450 g
- Language English 700
Categories
Long description:
Combinatorial Biotechnology: Methods for Synthesis of Peptide, Oligonucleotide and Oligosaccharide Libraries and Applications covers methods used for synthesizing peptide, oligonucleotide, and oligosaccharide libraries. It presents the concepts of combinatorial biotechnology, detailing both solid phase and solution phase synthesis techniques, including modern biological methods such as in vitro and in vivo display libraries. Key topics include solid phase synthesis, parallel peptide synthesis, encoding technologies, deconvolution methods, and chemical and biological ligations. With chapters covering the chemical and biological synthesis of recombinant peptides and proteins, as well as the genetic reprogramming of the genetic code, this book offers a rich source of practical guidance.
The inclusion of advanced methods and illustrative examples makes it a unique and essential reference in the field. This book is an essential resource for undergraduate and postgraduate students in biochemical engineering, biotechnology, and biosciences, as well as industry professionals in R&D divisions. It equips readers with the latest techniques and applications in combinatorial biotechnology, addressing current challenges and advancing their research and development efforts.
Table of Contents:
1. Solid Phase synthesis
2. Solid phase peptide synthesis
3. Solid phase Oligonucleotide synthesis
4. Solid phase oligosaccharide synthesis
5. Parallel peptide synthesis on solid phase Parallel synthesis: microplate method, Tea bag approach, Multi pin approach, SPOT synthesis, Light directed parallel synthesis, parallel synthesis using micro fluidic devices, peptide synthesis using peptide laser printer, cLIFT
6. Split only approach for peptide synthesis
7. Split and mix approach for peptide synthesis
8. Encoding Technologies Peptide encoding, Shaving, Oligonucleotide encoding, PNA encoding, Binary encoding, Mass encoding, optical encoding using fluorescent colloids, internal encoding, Physical encoding, electronic encoding, Graphical encoding.
9. Solution phase synthesis Dendrimers, Polymer supported reagents, scavenger resins, Fluorous reactants
10. Encoding in solution phase
11. Deconvolution methods Iterative deconvolution, Positional scanning, Orthogonal deconvolution, Subtractive deconvolution, Bogus coin deconvolution, Recursive deconvolution
12. Chemical Ligations Native Chemical ligation, Intein mediated ligation, Staudinger ligation
13. Biological methods: In vivo Display Libraries Filamentous phage display, lambda phage display, Bacterial cell surface display, Yeast cell surface display, Baculovirus capsid display, Mammalian cell surface display
14. Biological methods: In vitro display libraries Ribosomal display, mRNA display, CIS/CAD display, Plasmid display, In vitro compartmentalization
15. Genetic code reprogramming
