Basic Cell Culture
Series: Practical Approach Series; 254;
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Product details:
- Edition number 2
- Publisher OUP Oxford
- Date of Publication 10 January 2002
- ISBN 9780199638536
- Binding Paperback
- No. of pages408 pages
- Size 245x189x22 mm
- Weight 761 g
- Language English
- Illustrations numerous figure and halftones 0
Categories
Short description:
This book is a guide for the newcomer to all those areas which are currently basic to the performance of animal (including human) cell culture. Experienced workers should also find it useful as a source of reference when entering a new field, or when setting up a new laboratory.
MoreLong description:
The use of animal, including human, cell culture has expanded enormously during the last 25 years, with new applications appearing all the time. The aim of this book is to guide the newcomer progressively through all those areas which nowadays are basic to the performance of cell culture. The book should also prove useful to the experienced worker when entering a new field, setting up a new laboratory, or as a source of reference on basic techniques.
Revised and updated chapters cover topics such as equipping a cell culture laboratory, sterilization, culture media, basic culture technique, cell cloning, the isolation of new cell lines, contamination procedures, and good laboratory practice. There is a brand new chapter on the microscopy of living cells, and an appendix of online resources for cell biology.
"I think that this is a very useful book, and I recommend it not only to beginners, but also to more experienced cell culture workers, who will find many new hints and pieces of information, presented in a readily accessible way."
Table of Contents:
The Cell Culture Laboratory
Sterilization
Culture Media
Microscopy of Living Cells
Basic cell culture and the maintenance of cell lines
Primary culture and the establishment of cell lines
Specific cell types and their requirements
Cloning
The quality control of cell lines
Good laboratory practice in the cell culture laboratory
Online resources for cell biology
