Advanced Protocols in Cancer Research by Arunachalam, Karuppusamy; Sreeja, Puthanpura Sasidharan; Anand, Krishnan;

Long description:

This detailed volume navigates the journey from a promising chemical entity to a potential therapeutic agent in cancer research through systematic and rigorous protocols across a spectrum of increasingly sophisticated models. Beginning with foundational in vitro assays for assessing cytotoxicity and antiproliferative effects, the book continues by covering mechanisms of action studies, advanced 2D and 3D culture models for the tumor microenvironment and cellular architecture involved in modulating drug response and resistance, in vivo antitumor and efficacy models, as well as immunology and inflammation-related assays and forward-looking clinical studies for cancer research. Written for the Springer Protocols Handbooks series, chapters include the kind of precise implementation advice and methodological clarity that leads to success in advancing the current state of the research.

Comprehensive and practical, Advanced Protocols in Cancer Research: Translational Models for Antitumor and Anticancer Drug Discovery serves as a valuable resource for fostering methodological precision and accelerating the translation of scientific discovery into effective cancer therapies.

Table of Contents:

In Vitro Cytotoxicity Assays: The MTT Assay.- Alamar Blue Cytotoxicity Assay: Protocol for Tumor Cell Lines and Peripheral Blood Mononuclear Cells (PBMCs).- Protocol for Evaluating the In Vitro Cytotoxicity of Compounds Against LM3 and LP07 Murine Adenocarcinoma Cell Lines Using the MTT Assay.- Evaluating the Cytotoxic and Anti-Cancer Mechanisms of Peptides on Tumor and Non-Tumor Cell Lines.- Comprehensive Assessment of Antiproliferative Activity and Topoisomerase Inhibition by Test Compounds in Cancer Cell Lines.- Evaluation of Cytotoxic Selectivity and Gene Expression Modulation by Novel Antitumor Compounds.- Evaluation of the Cytotoxic and Anti-Proliferative Effects of Phytocompound on Human Glioblastoma Cell Lines.- Hemolytic Activity Assay.- Evaluation of DNA Intercalation and Oxidative Cleavage by Bioactive Compounds.- Comprehensive Analysis of Oxidative Stress Markers in Tumor Ascitic Exudate from a Murine Model.- Evaluation of Oxidative Stress Markers: Experimental Procedure.- In Vitro Evaluation of Cytotoxicity (Dark and Phototoxic), Cell Death Mechanism, and Confocal Imaging in NIH-3T3 and B16-F10 Cells.- Evaluation of Antiproliferative, Pro-Apoptotic, and Immunomodulatory Effects of Test Compounds on Human Leukemic Cell Lines and Primary Peripheral Blood Mononuclear Cells (PBMCs).- Advanced 2D and 3D Co-Culture Systems for Studying Breast Cancer Cell Interactions and GLS Inhibition.- In Vitro Breast and Cervical Cancer 2D and 3D Co-Culture Models.- Development of 3D Breast Cancer Spheroid Models and Evaluation of Paclitaxel Cytotoxicity in Monolayer Versus Spheroid Cultures.- Evaluation of Doxorubicin Efficacy on 2D and 3D Cultures of Triple-Negative Breast Cancer Cells (MDA-MB-231).- Generation and Analysis of SK-MEL-28 Melanoma Spheroids for 3D Co-Culture and Drug Testing Models.- In Vitro Development and Evaluation of Reconstructed Human Skin Models Using HaCat, MRC5, and CAL27 Cell Lines.- Multi-Elemental Analysis Using X-Ray Microfluorescence in 3D Cultures of Human Neuroblastoma Cells.- Integrated Computational and Cellular Analysis of Triterpenoid Compounds: Target Prediction, Pharmacokinetic Profiling, and Antimelanoma Activity in 2D and 3D Models.- In Vitro Evaluation of Biflorin’s Antitumor Activity.- In Vitro Evaluation of Cytotoxic Activity and Mechanisms of Action of Compounds in Human Cancer Cell Lines.- Evaluation of Antitumor Potential of Phytocompound Fractions.- Comprehensive Protocol for Evaluating Antiproliferative and Pro-Apoptotic Effects of Novel Compounds.- In Vivo Biological Assays.- Evaluation of Antitumor Action in Sarcoma 180 Solid Tumor Model (In Vivo).- Evaluation of the Antitumor Activity in HL-60 Cells and TRAMP Mouse Model.- In Vivo Evaluation of Antitumor and Antimetastatic Activity Using Syngeneic and Xenograft B16F10 Melanoma Models.- Evaluation of Isoquercetin and Rutin in a Human Colon Adenocarcinoma Xenograft Model in Balb/c Nude Mice.- Comprehensive Preclinical Evaluation of Anticancer Compounds: Cytotoxicity, Apoptosis Induction, and In Vivo Efficacy.- Comprehensive Protocol for In Vitro and In Vivo Antitumor Evaluation of Phytocompounds in the Ehrlich Ascites Tumor Model.- In Vivo Assay for Leukocyte Migration, Exudation, and Myeloperoxidase (MPO) Activity Using the Murine Air Pouch Model.- Isolation and In Vitro Differentiation of Bone Marrow-Derived Macrophages (BMDMs).- Macrophage Activation Quantified via Nitric Oxide (NO) Griess Assay.- Cellular Internalization and Localization of Fluorescein Isothiocyanate (FITC)-Labeled Peptide via Confocal Microscopy.- Annexin V-FITC/Propidium Iodide (PI) Flow Cytometry Apoptosis Assay.- Transmission Electron Microscopy (TEM) for Ultrastructural Analysis of Apoptosis.- Detection of Intracellular Superoxide Anion Production Using Dihydroethidium (DHE).- MTT Cell Viability Assay to Test Protective Effect of N-Acetylcysteine (NAC).- Lymphocyte Isolation Protocol from Human Peripheral Blood.- Circulating Tumor Cell (CTC) Isolation Protocol.- Exosome Isolation Protocol from Blood.