A Practical Guide to Protein and Peptide Purification for Microsequencing
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Product details:
- Edition number 2
- Publisher Academic Press
- Date of Publication 22 July 1993
- ISBN 9780124802827
- Binding Paperback
- No. of pages184 pages
- Size 228x152 mm
- Weight 290 g
- Language English 0
Categories
Long description:
Why a Second Edition?
The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.
Table of Contents:
Strategies for Obtaining Partial Amino Acid Sequence Data from Small Quantities (5nmol) of Pure of Partially Purified Protein. Enzymatic Digestion of Proteins and HPLC Peptide Isolation
Purification of Proteins and Peptides By SDS-PAGE
Internal Amino Acid Sequence Analysis of Proteins After in Situ Protease Digestion on Nitrocellulose
Mass Spectrometric Strategies for the Structural Characterization of Proteins
