A Practical Guide to Developmental Biology
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Product details:
- Publisher OUP Oxford
- Date of Publication 21 August 2003
- ISBN 9780199249718
- Binding Paperback
- No. of pages128 pages
- Size 245x188x8 mm
- Weight 287 g
- Language English
- Illustrations numerous line figures and diagrams 0
Categories
Short description:
This lab manual is designed to give students experience with a wide variety of model systems currently in use by developmental biologists. Experiments range from classic slide or whole animal observations to more modern immunohistochemistry and manipulation of gene expression. All of these experiments can be completed on a relatively small budget.
MoreLong description:
This lab manual is designed for upper level undergraduates or graduate students, to introduce them to the field of developmental biology. After spending two weeks learning how to handle and manipulate a variety of embryonic organisms, students will begin a series of experiments that more or less keep pace with the sequence of most developmental biology textbooks (axial patterning, plant cell totipotency, fertilization, early plant development, morphogenesis, cell adhesion, embryogenesis, gametogenesis, regeneration and metamorphosis. The manual is heavily illustrated and gives students a solid grounding in classic developmental biology as well as modern techniques in immunohistochemistry and homeobox gene expression. Appendices of recipes, needed chemicals, and sources for animals are included.
All in all a clearly written laboratory aid that should prove highly useful to those involved in developmental biology.
Table of Contents:
Table of Contents
The Experiments
Introduction to Developmental Biology: Embryo Protocols, Ethics and Model Systems
Axial Patterning: Using Retinoic Acid to Disrupt Homeobox Gene Expression in Axolotls
Plant Cell Totipotency: Growing a Carrot from Adult Cells
Fertilization: Sea Urchin Syngamy & Development
Early Plant Development: Pollen Tube Formation
Morphogenesis: Creating Fate Maps of Albino Axolotls Using a Vital Dye
Cell Adhesion: Cell-Cell Interactions in Sponges & Amphibians
Embryogenesis: Chick & Amphibian Development (Slide-based)
Neurulation: Labelling Chick Notochord using Immunocytochemistry
Gametogenesis: Comparison of Plants and Animals
Regeneration: Morphallaxis & Epimorphosis
Metamorphosis: Drosphila Imaginal Discs
External Influences on Development: Design Your Own Experiment with Amphibians, Chicks or Fish
Information for the Instructor
Animal Care & Maintenance
Animal & Plant Sources
Chemical Sources
Slides Needed / Sources
Solutions & Other Materials
Glossary
Literature Cited
