Skeletal Development and Repair
EUR 181.89
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| ISBN13: | 9781071610275 |
| ISBN10: | 1071610279 |
| Kötéstípus: | Keménykötés |
| Terjedelem: | 482 oldal |
| Méret: | 254x178 mm |
| Súly: | 1128 g |
| Nyelv: | angol |
| Illusztrációk: | 20 Illustrations, black & white; 105 Illustrations, color |
| 263 |
This second edition provides a comprehensive laboratory manual on skeletal development and skeletal repair research utilizing mouse models. Chapters provide methods and protocols on the most current and cutting-edge techniques in skeletal development and repair, histological, cellular, and molecular techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Skeletal Development and Repair: Methods and Protocols-Second Edition aims to ensure successful results in the further study of this vital field.
This second edition provides a comprehensive laboratory manual on skeletal development and skeletal repair research utilizing mouse models. Chapters provide methods and protocols on the most current and cutting-edge techniques in skeletal development and repair, histological, cellular, and molecular techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Skeletal Development and Repair: Methods and Protocols-Second Edition aims to ensure successful results in the further study of this vital field.
Part I. Murine Skeletal Development, Repair, and Genetic Models
1. Overview of Skeletal Development
Tatsuya Kobayashi and Henry M. Kronenberg
2. Overview of Skeletal Repair (Fracture Healing and Its Assessment)
Elise F. Morgan, Anthony De Giacomo, and Louis C. Gerstenfeld
3. Advantages and Limitations of Cre Mouse Lines Used in Skeletal Research
Florent Elefteriou and Greig Couasnay
Part II. Skeletal Repair, Parabiosis, Transplantations, and Organ Cultures
4. Generation of Closed Transverse Fractures in Small Animals
Anthony De Giacomo, Elise F. Morgan, and Louis C. Gerstenfeld
5. A Mouse Femoral Ostectomy Model to Assess Bone Graft Substitutes
Ryan Trombetta, Emma K. Knapp, and Hani A. Awad
6. Surgical Induction of Posttraumatic Osteoarthritis in the Mouse
Robert D. Maynard, David A. Villani, William G. Schroeder, Douglas J. Adams, and Michael J. Zuscik
7. Parabiosis: Assessing the Effects of Circulating Cells and Factors on the Skeleton
Benjamin Aaron Alman and Gurpreet Singh Baht
8. Murine Limb Bud Organ Cultures for Studying Musculoskeletal Development
Martin Arostegui and T. Michael Underhill
9. Murine Limb Explant Cultures to Assess Cartilage Development
Manuela Wuelling and Andrea Vortkamp
10. Renal Capsule Transplantation to Assay Angiogenesis in Skeletal Development and Repair
Anais Julien, Simon Perrin, Rana Abou-Khalil, and Céline Colnot
Part III. Structural, Histological, and Molecular Analyses on Skeletal Tissues and Tissue Sections
11. MicroCT for Scanning and Analysis of Mouse Bones
Yung Kim, Michael D. Brodt, Simon Y. Tang, and Matthew J. Silva
12. Four-Point Bending Testing for Mechanical Assessment of Mouse Bone Structural Properties
Hattie C. Cutcliffe and Louis E. DeFrate
13. Phenotyping Intact Mouse Bones using Bone CLARITY
Jennifer B. Treweek, Aidan Beres, Nathan Johnson, and Alon Greenbaum
14. Processing and Sectioning Undecalcified Murine Bone Specimens
Thomas B. Bemenderfer, Jonathan S. Harris, Keith W. Condon, Jiliang Li, and Melissa A. Kacena
15. Preparation of Thin Frozen Sections from Nonfixed And Undecalcified Hard Tissues Using Kawamoto's Film Method (2020)
Tadafumi Kawamoto and Komei Kawamoto
16. Demineralized Murine Skeletal Histology
Anthony J. Mirando and Matthew J. Hilton
17. Assessment of Osteocytes: Techniques for Studying Morphological and Molecular Changes Associated With Perilacunar/ Canalicular Remodeling Of the Bone Matrix
Neha S. Dole, Cristal S. Yee, Charles A. Schurman, Sarah L. Dallas, and Tamara Alliston
18. Cell Lineage Tracing: Co-localization of Cell Lineage Markers with a Fluorescent Reporter
Yan Jing, Patricia Simmer, and Jian Q. Feng
19. Immunofluorescent Staining of Adult Murine Paraffin-embedded Skeletal Tissue
Neta Felsenthal and Elazar Zelzer
20. Detection of Hypoxic Regions in the Bone Microenvironment
Wendi Guo and Colleen Wu
21. EdU-based Assay of Cell Proliferation and Stem Cell Quiescence in Skeletal Tissue Sections
Marco Angelozzi, Charles R. de Charleroy, and Véronique Lefebvre
22. Whole-mount In Situ Hybridization in Murine Tissues
Deepika Sharma, Matthew J. Hilton, and Courtney M. Karner
Part IV. Primary Cell Isolations, Cultures, and Assays
23. Bone Marrow Stromal Cell Assays ? In Vitro and In Vivo
Pamela Gehron Robey, Sergei A. Kuznetsov, Paolo Bianco, and Mara Riminucci
24. Isolation and Culture of Periosteum Derived Progenitor Cells from Mice
Chinedu C. Ude, Girdhar G. Sharma, Jie Shen, and Regis J. O?Keefe
25. Isolation and Culture of Murine Primary Chondrocytes: Costal and Growth Plate Cartilage
Yihan Liao, Jason T. Long, Christopher J.R. Gallo, Anthony J. Mirando, and Matthew J. Hilton
26. Isolation and Culture of Neonatal Mouse Calvarial Osteoblasts
Madison L. Doolittle, Cheryl L. Ackert-Bicknell, and Jennifer H. Jonason
27. Mitochondrial Function and Metabolism of Cultured Skeletal Cells
Li Tian, Clifford J Rosen, and Anyonya R Guntur
28. Radiolabeled Amino Acid Uptake Assays in Primary Bone Cells and Bone Explants
Leyao Shen and Courtney M. Karner
29. RANKL-based Osteoclastogenic Assay from Murine Bone Marrow Cells
Zhenqiang Yao, Lianping Xing, and Brendan F. Boyce
30. Hematopoietic Stem Cell Cultures and Assays
Benjamin J. Frisch
