Methods in Cyanobacterial Research

 
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Kiadó: CRC Press
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Rövid leírás:

This laboratory reference compiles the essential protocols used in cyanobacterial research. Cyanobacteria is a model system for biofuel production and sequestration of carbon dioxide to tackle global climate change due to increasing levels of greenhouse gases.

Hosszú leírás:

This laboratory reference compiles the essential protocols used in cyanobacterial research. Cyanobacteria is a model system for biofuel production and sequestration of carbon dioxide to tackle global climate change due to increasing levels of greenhouse gases. Topics range from identification and growth parameters to biomolecule estimation and isolation. It also covers genome analysis and bioengineering of cyanobacteria for novel products.


Key Features:



  • Provides step-by-step procedures and troubleshooting tips

  • Covers identification, growth parameters and pigment estimation methodologies

  • Describes isolation, characterization and purification of microcystin, phycobiliproteins and scytonemin from cyanobacteria

  • Discusses genomics, proteomics and bioengineering of cyanobacteria from novel products

  • Explains sample preparation and visualization protocol for electron microscopy-based analysis of cyanobacteria

This collection is useful to students and researchers in life sciences. It is also meant for industry experts who are involved in the production of biofuels, biofertilizers, other value-added products, and carbon sequestration using cyanobacteria.

Tartalomjegyzék:

Chapter 1: Evaluating the polyphasic approach in cyanobacterial taxonomy


Chapter 2: Estimation of growth and photosynthetic pigments in cyanobacteria


Chapter 3: Quantitative estimation of total carbohydrate, protein and lipid contents in cyanobacteria


Chapter 4: Isolation and purification of phycobiliproteins


Chapter 5: Methods for estimation of oxidative stress indices in cyanobacteria


Chapter 6: Estimation of glutamine synthetase enzyme


Chapter 7: Estimation of nitrate reductase enzyme


Chapter 8: Estimation of nitrogenase enzyme activity in cyanobacteria


Chapter 9: Isolation and purification of cyanobacterial toxins


Chapter 10: Isolation and purification of scytonemin


Chapter 11: Isolation and purification of mycosporine-like amino acids


Chapter 12: Protein estimation in cyanobacterial samples


Chapter 13: Proteomic analysis of cyanobacteria employing 2-dimensional gel electrophoresis (2-DE)


Chapter 14: LC-MS/MS based proteomics study of cyanobacteria


Chapter 15: Monitoring photosynthetic performance in cyanobacteria by a modulated fluorometer


Chapter 16: Reactive oxygen species measurement in cyanobacteria using 2?,7?- dichlorodihydrofluorescein diacetate (DCFH-DA) dye and fluorescence microscope or flow cytometer


Chapter 17: Flow cytometry-based methods for estimating DNA content and live-dead cells in cyanobacteria


Chapter 18: Genomic DNA and RNA extraction from cyanobacteria


Chapter 19: Detection and quantification of DNA damage in cyanobacteria by immunodot- blot assay


Chapter 20: Engineering cyanobacteria for the synthesis of novel products


Chapter 21: Sample preparation and visualization protocol for electron microscopy-based analysis of cyanobacteria


Chapter 22: Cyanobacterial biofertilizer production technology


Chapter 23: Biomass composition measurement and intracellular flux analyses for insights into cyanobacterial metabolism


Chapter 24: In silico genome assembly and functional annotation


Index