Kidney Research
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| ISBN13: | 9781071631782 |
| ISBN10: | 10716317811 |
| Kötéstípus: | Keménykötés |
| Terjedelem: | 353 oldal |
| Méret: | 254x178 mm |
| Súly: | 896 g |
| Nyelv: | angol |
| Illusztrációk: | 11 Illustrations, black & white; 76 Illustrations, color |
| 627 |
This third edition provides updated and new chapters on recent innovations in basic renal research. Chapters are divided into three sections covering in vitro, in vivo, and ex vivo models of kidney disease, recent advances in imaging techniques and protocols for performing analytical and functional measurements in the kidney; techniques that are both topical and of widespread relevance to the study of kidney biology and disease. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Kidney Research: Experimental Protocols, Third Edition aims to be comprehensive guide for researchers in the field.
This third edition provides updated and new chapters on recent innovations in basic renal research. Chapters are divided into three sections covering in vitro, in vivo, and ex vivo models of kidney disease, recent advances in imaging techniques and protocols for performing analytical and functional measurements in the kidney; techniques that are both topical and of widespread relevance to the study of kidney biology and disease. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Kidney Research: Experimental Protocols, Third Edition aims to be comprehensive guide for researchers in the field.
Part I: In vitro, in vivo and ex vivo models of kidney disease
1. Restoration of podocyte phenotype in culture
Eishin Yaoita
2. Protocols for the propagation, culture and characterization of kidney fibroblasts
Tim D. Hewitson and Edward R. Smith
3. Isolation of rat glomeruli and propagation of mesangial cells to study the kidney in health and disease
Tim D. Hewitson and Edward R. Smith
4. In vitro models to evaluate molecular permeability of the kidney filtration barrier
Dan Wang and Nicholas Ferrell
5. Generating human glomeruli from pluripotent stem cells for disease modelling and drug screening
Lorna J. Hale and Melissa H. Little
6. Large-scale production of kidney organoids from human pluripotent stem cells
Veronika Sander, Aneta Przepiorski, Neil A. Hukriede, and Alan J. Davidson.
7. Construction of a multitubular perfusable kidney-on-chip for the study of renal disease
Brice Lapin, Sarah Myram, Manh-Louis Nguyen, Giacomo Gropplero, Sylvie Coscoy, and Stéphanie Descroix
8. A ?kidney-on-the-chip? model composed of primary human tubular, endothelial, and white blood cells
Julian A. Marschner, Lucas Martin, Gregor Wilken, Maria Elena Melica, and Hans-Joachim Anders
9. Standardized protocol for the preparation of precision-cut kidney slices: a translational model of renal fibrosis
Michael Schou Jensen, Camilla Merrild, Rikke N?rregaard, Peter Olinga, and Henricus A.M. Mutsaers
10. Culture of three-dimensional Madin-Darby Canine Kidney (MDCK) cysts for in vitro drug testing in polycystic kidney disease
Sayanthooran Saravanabavan and Gopala. K. Rangan
11. Zebrafish model to study podocyte function within the glomerular filtration barrier
Nina Sopel and Janina Müller-Deile
Part II: Imaging in kidney research
12. Magnetic Resonance Imaging (MRI) analysis of tissue sodium concentration in chronic kidney disease
Kylie Martin, Vijay Venkatraman, Angela Agostinelli, Braden Thai, Daniel Stäb, Tim D. Hewitson, Sven-Jean Tan, Nigel D. Toussaint, and Patsy Robertson
13. Determination of interstitial collagen deposition and related topography using the second harmonic generation-based Histoindex platform
Sadman Bhuiyan, Robert E. Widdop, and Chrishan S. Samuel
14. Quantitative imaging of podocyte foot processes in the kidney using confocal and STED microscopy
David Unnersjö-Jess
15. Synthesis and expression of a targeted, ferritin-based tracer for PET imaging of kidney glomeruli
Edwin J. Baldelomar, Kasey C. Emoto, Maria Veronica Clavijo Jordan, Jennifer R. Charlton, David E. Reichert, Neda Parvin, Courtnie Yokono, Bradley D. Hann, and Kevin M. Bennett
Part III: Analytical and functional measurements in the kidney
16. Multiplex in situ hybridization in the study of acute kidney injury
Agnieszka Masztalerz, Alex Gregorieff, Serge Lemay, and Tomoko Takano
17. Spatial transcriptomics in kidney tissue
Arti M. Raghubar, Joanna Crawford, Kahli Jones, Pui Y. Lam, Stacey B. Andersen, Nicholas A Matigian, Monica S.Y. Ng, Helen Healy, Andrew J. Kassianos, and Andrew J. Mallett
18. Assessment of kidney mitochondrial function by high-resolution respirometry, transmission electron microscopy and histological techniques
Marisol Orozco-Ibarra, Omar Emiliano Aparicio-Trejo, Alexis Paulina-Jiménez Uribe, Estefani Yaquelin Hernández-Cruz, Ana Karina Aranda-Rivera, Isabel Amador- Martínez, Francisca Fernández-Valverde, and José Pedraza-Chaverri
19. Transdermal measurement of glomerular filtration rate in preclinical research
Thomas P Mullins, Daniel Schock-Kusch, and Linda A Gallo
20. The BioHybrid Assay ? a novel method for determining calcification propensity
Armand M.G. Jaminon, Asim C. Akbulut, Chris P. Reutelingsperger, and Leon J. Schurgers
21. Quantification of calciprotein particles (CPPs) in serum/plasma samples using a fluorescent bisphosphonate
Yutaka Miura, Hiroshi Kurosu, Makoto Kuro-o
22. Monitoring of Rho GTPase Activity in Podocytes
Jun Matsuda and Tomoko Takano
