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    Enzymes in RNA Science and Biotechnology Part B

    Enzymes in RNA Science and Biotechnology Part B by Yamagami, Ryota; Kwok, Chun Kit;

    Sorozatcím: Methods in Enzymology; 692;

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        60 236 Ft (57 368 Ft + 5% áfa)
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    A termék adatai:

    • Kiadó Academic Press
    • Megjelenés dátuma 2023. november 6.

    • ISBN 9780443239762
    • Kötéstípus Keménykötés
    • Terjedelem346 oldal
    • Méret 228x152 mm
    • Súly 700 g
    • Nyelv angol
    • 552

    Kategóriák

    Hosszú leírás:

    Enzymes in RNA Science and Biotechnology, Part B, Volume 692 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on topics such as Quantitative base-resolution sequencing technology for mapping pseudouridines in mammalian mRNA, Quantitative base-resolution DAMM-seq for mapping RNA methylations in tRNA and mitochondrial dsRNA, Discovering RNA modification enzymes using a comparative genomics approach, Functional analysis of tRNA modification enzymes using mutational profiling, and Fluorescent labeling of tRNA for rapid kinetic interaction studies with tRNA-binding proteins.

    Other chapters cover Enzymatic Synthesis of RNA Standards for Mapping and Quantifying RNA Modifications in Sequencing Analysis, Characterizing RNase L-mediated mRNA decay in single cells, Characterization of RNase J, Pri-miRNA cleavage assays for the Microprocessor complex, The pre-miRNA cleavage assays for DICE, Methods for study of ribonuclease targeting chimeras, and Profiling the in vivo RNA interactome associated with the endoribonuclease RNase III in Staphylococcus aureus.

    Több

    Tartalomjegyzék:

    Post-transcriptional modification enzymes
    1. Quantitative base-resolution sequencing technology for mapping pseudouridines in mammalian mRNA
    Li-Sheng Zhang
    2. Quantitative base-resolution DAMM-seq for mapping RNA methylations in tRNA and mitochondrial dsRNA
    Li-Sheng Zhang
    3. Discovering RNA modification enzymes using a comparative genomics approach
    Satoshi Kimura
    4. Functional analysis of tRNA modification enzymes using mutational profiling
    Ryota Yamagami and Hiroyuki Hori
    5. Fluorescent labeling of tRNA for rapid kinetic interaction studies with tRNA-binding proteins
    Ute Kothe and Sarah Schultz
    6. Enzymatic Synthesis of RNA Standards for Mapping and Quantifying RNA Modifications in Sequencing Analysis
    Howard Gamper, Caroline McCormick, Amr Makhamreh, Meni Wanunu, Sara Rouhanifard and Ya-Ming Hou

    Nucleases
    7. Characterizing RNase L-mediated mRNA decay in single cells
    James Burke, Renee Cusic and Monty Watkins
    8. Characterization of RNase J
    Balasubramanian Gopal, Muralidharan Vandanashree and Ankur Singh
    9. Pri-miRNA cleavage assays for the Microprocessor complex
    Tuan Anh Nguyen, Cong Truc Le and Thi Nhu Y. Le
    10. The pre-miRNA cleavage assays for DICER
    Tuan Anh Nguyen, Cong Truc Le and Thi Nhu Y. Le
    11. Methods for study of ribonuclease targeting chimeras
    Matt Disney, Noah Springer, Samatha Meyer, Yuquan Tong, Jessica Childs-Disney and Raphael Isaac Benhamou
    12. Profiling the in vivo RNA interactome associated with the endoribonuclease RNase III in Staphylococcus aureus
    Winton Wu, Chi Nam Ignatius Pang, Jai Tree and Daniel Giuseppe Mediati

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