| ISBN13: | 9781071609071 |
| ISBN10: | 1071609076 |
| Kötéstípus: | Keménykötés |
| Terjedelem: | 334 oldal |
| Méret: | 254x178 mm |
| Súly: | 852 g |
| Nyelv: | angol |
| Illusztrációk: | 12 Illustrations, black & white; 69 Illustrations, color; 72 Tables, color |
| 230 |
DNA Cloning and Assembly
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This volume provides a comprehensive collection of DNA assembly protocols that will prove useful for any researcher interested in molecular cloning, synthetic biology, or DNA manipulation. Chapters will guide readers through computational tools to design and track the construction of DNA assemblies, workflows that enable high-throughput assembly of DNA constructs, standardized toolkits and protocols for DNA assembly, and combinatorial solutions that enable the construction and optimization of entire metabolic pathways. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, DNA Cloning and Assembly: Methods and Protocols aims to ensure successful results in the further study of this vital field.
This volume provides a comprehensive collection of DNA assembly protocols that will prove useful for any researcher interested in molecular cloning, synthetic biology, or DNA manipulation. Chapters will guide readers through computational tools to design and track the construction of DNA assemblies, workflows that enable high-throughput assembly of DNA constructs, standardized toolkits and protocols for DNA assembly, and combinatorial solutions that enable the construction and optimization of entire metabolic pathways. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, DNA Cloning and Assembly: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Part I: In Silico Tools for DNA Design and Assembly
1. An Integrated Computer-Aided Design and Manufacturing Workflow for Synthetic Biology
Ernst Oberortner, Robert Evans, Xianwei Meng, Sangeeta Nath, Hector Plahar, Lisa Simirenko, Angela Tarver, Samuel Deutsch, Nathan J. Hillson, and Jan-Fang Cheng
2. Combinatorial-Hierarchical DNA Library Design Using the TeselaGen DESIGN Module with j5
Michael J Fero, James K Craft, Trang Vu, and Nathan J Hillson
3. The AssemblX Toolkit for Reliable and User-friendly Multi-gene Assemblies
Fabian Machens and Lena Hochrein
Part II: High Throughput Workflows
4. High-Throughput PCR for DNA Part Generation
David Reif
5. High-Throughput DNA Assembly Using Yeast Homologous Recombination
Kristy Ip, Ron Yadin, and Kevin W. George
6. Highly-Multiplexed, Semi-Automated Nextera Next Generation Sequencing (NGS) Library Preparation
William Christie, Ron Yadin, Kristy Ip, and Kevin W. George
Part III: DNA Assembly Standards
7. Generation of MoClo Standard Parts Using Golden Gate Cloning
Ramona Grützner and Sylvestre Marillonnet
8. Assembly of Multigene Constructs using the Modular Cloning System MoClo
Sylvestre Marillonnet and Stefan Werner
9. Hierarchical Modular DNA Assembly using MetClo
Da Lin and Christopher A. O?Callaghan
10. PaperClip DNA Assembly: Reduce, Reuse, Recycle
Maryia Trubitsyna, Annegret Honsbein, Uma Jayachandran, Alistair Elfick, and Christopher E. French
11. Phytobricks: Manual and Automated Assembly of Constructs for Engineering Plants
Yao-Min Cai , Jose A. Carrasco Lopez, and Nicola J. Patron
12. Mobius Assembly
Andreas I. Andreou and Naomi Nakayama
13. Design and Implementation of Multi-Protein Expression Constructs and Combinatorial Libraries using Start-Stop Assembly
George M. Taylor and John T. Heap
14. BASIC ? A Simple and Accurate Modular DNA Assembly Method
Marko Storch, Ari Dwijayanti, Haris Mallick, and Matthew C. Haines, and Geoff S. Baldwin
15. Joint Universal Modular Plasmids: A Flexible Platform for Golden Gate Assembly in Any Microbial Host
Marcos Valenzuela-Ortega and Christopher E. French
Part IV: Combinatorial Methods For Pathway Optimization
16. A Step-by-Step Protocol for COMPASS, a Synthetic Biology Tool for Combinatorial Gene Assembly
Gita Naseri and Bernd Mueller-Roeber
17. SCRaMbLE-in: A Fast and Efficient Method to Diversify and Improve the Yields of Heterologous Pathways in Synthetic Yeast
Reem Swidah, Jamie Auxillos, Wei Liu, Sally Jones, Ting-Fung Chan, Junbiao Dai, and Yizhi Cai
