Wheat Rusts and Resistance Breeding by Brar, Gurcharn Singh; Holden, Samuel;

Long description:

This detailed volume collects techniques for increasing our understanding of wheat rust pathogens, the host plant, and the interactions between the two. Beginning with routine and advanced tools used for pathogen surveillance, characterization, and field assessment of germplasm for resistance, the book continues with sections on genetic analysis and breeding for rust resistance, as well as rust management through fungicidal approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Wheat Rusts and Resistance Breeding: Methods and Protocols serves as an ideal guide for established researchers looking to familiarize themselves with new innovations and for new researchers working to strengthen their understanding of rust pathogens.

Table of Contents:

Race Characterization of Puccinia triticina, the Causal Agent of Wheat Leaf Rust, in Canada.- Race Characterization of Puccinia striiformis f. sp. tritici and P. striiformis f. sp. hordei in the United States.- Stripe Rust Virulence and Host Reaction Assessment Protocols: From Lab to Field.- Characterizing Pathotypes of Puccinia striiformis and Their Impact in Agriculture in Australia.- Integrated Approach for Genotyping and Race Typing of Puccinia striiformis in Wheat.- MARPLE: Mobile and Real Time Plant Disease Diagnostics for Enhancing Wheat Rust Surveillance.- Microscopic Analyses of Rust Species: Preparation of Samples and Slides.- Identifying Wheat Rusts Using MALDI-TOF Mass Spectrometry.- High Molecular Weight DNA Extraction from Wheat Rust Spores.- Identification of Rust Fungi Using High-Throughput Sequencing Data from Environmental Samples.- A Wheat Protoplast Assay for Positive Effector Screening and Investigation of Host-Pathogen Interactions.- Field Phenotyping of Wheat Leaf Rust and Stem Rust.- Field Scoring of Wheat Yellow Rust Disease Under Field Conditions: Germplasm Evaluation and Farmer Field Surveys.- Field Phenotyping of Wheat Stripe Rust and Leaf-Tip Necrosis Associated with Rust Resistance Genes.- Identification and Genetic Analysis of Rust Resistance Genes in Wheat for Marker-Assisted Selection.- QTL/Linkage-Mapping for Rust Resistance: Step-by-Step Guide.- Developing Breeder-Friendly KASP Markers for Rust R Genes.- Development of a High-Resolution Melt (HRM) Marker Diagnostic to the Wheat (Triticum aestivum L.) Leaf Rust Resistance Gene Lr34.- Sequencing Trait-Associated Mutations (STAM) to Clone Rust Resistance Genes.- A Protocol for High-Efficiency Transformation and Genome Editing in Elite Wheat Cultivars.- Production of Doubled Haploid Spring Wheat Plants: Wheat x Maize Hybridization and Microspore Culture Methods.- In Vitro and In Planta Fungicide Testing for Management of Wheat Rusts.