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Product details:
- Edition number 2
- Publisher Oxford University Press
- Date of Publication 14 October 1999
- Number of Volumes laminated boards
- ISBN 9780199637294
- Binding Hardback
- No. of pages460 pages
- Size 241x162x28 mm
- Weight 917 g
- Language English
- Illustrations 9 halftones, numerous line illustrations 0
Categories
Short description:
Reversible protein phosphorlyation is one of the major mechanisms of controlling protein activity. This new edition provides a comprehensive description of current methods used to investigate this important phenomenon.
MoreLong description:
Reversible phosphorylation is one of the major mechanisms of controlling protein activity in all eukaryotic cells. This new edition of Protein Phosphorylation: A Practical Approach provides a comprehensive description of current methods used to study protein phosphorylation and the kinases and phosphatases which catalyse it. It includes protocols for studying phosphorylation in intact cells; analysis of signal transduction pathways, kinase specificity, and
kinase interactions; assay and purification of kinases and phosphatases; and identification of substrates. Also covered are cloning and expression protocols and advice on the crystallization of kinases and phosphatases. Protein Phosphorylation: A Practical Approach 2e will therefore be of great value to any
researcher investigating aspects of reversible protein phosphrylation.
"This will be an invaluable laboratory aid to researches involved in this field" Aslib Book Guide
Table of Contents:
Preface
Study of protein phosphorylation in intact cells
Analysis of signal transduction pathways using protein kinase inhibitors and activators
Analysis of signal transduction pathways using proteinserine/threonine phosphatase inhibitors
Analysis of signal transduction pathways using molecular biological manipulations
Phosphopeptide mapping and phosphoamino acid analysis on cellulose thin-layer plates
Phosphorylation site analysis by mass spectrometry
Assay and purification of protein (serine/threonine) phosphatases
[chapter title missing]
Identification of substrates for protein-tyrosine phosphatases
Assay and purification of protein-serine/threonine kinases
Assay of protein kinases and phosphatases using specific peptide substrates
Cloning and expression of cDNAs encoding protein kinase subunits
Interaction cloning of protein kinase partners
Identifying protein kinase substrates by expression screening with solid-phase phosphorylation
Analysis of protein kinase interactions using the two-hybrid method
Analysis of protein kinase interactions using biomolecular interaction analysis
Analysis of protein kinase specificity using orientated peptide libraries
Crystallization of protein kinases and phosphatases
Index
