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Product details:
- Publisher OUP Oxford
- Date of Publication 26 February 2004
- ISBN 9780199638734
- Binding Paperback
- No. of pages356 pages
- Size 246x189x19 mm
- Weight 715 g
- Language English
- Illustrations numerous figures, tables and one halftone 0
Categories
Short description:
This new book is designed to enable researchers to design and undertake all aspects of a phage display project, from designing an experimental strategy and constructing a library to performing selections and analyzing the results.All of the protocols and chapters are extensively cross-referenced, allowing readers to move beyond the specific examples provided in order to customize the procedures for their own protein or selection system of interest. Phage Display is an up-to-date, comprehensive and integrated experimental guide to the technique, which is essential reading for anyone currently using, or wishing to use the technique for basic research and drug discovery.
MoreLong description:
This new book is designed to enable researchers to design and undertake all aspects of a phage display project, from designing an experimental strategy and constructing a library to performing selections and analyzing the results.All of the protocols and chapters are extensively cross-referenced, allowing readers to move beyond the specific examples provided in order to customize the procedures for their own protein or selection system of interest. Phage Display is an up-to-date, comprehensive and integrated experimental guide to the technique, which is essential reading for anyone currently using, or wishing to use the technique for basic research and drug discovery.
MoreTable of Contents:
Introduction to phage biology and phage display
Constructing phage display libraries by oligonucleotide-directed mutagenesis
In vitro DNA recombination
Phage selection strategies for improved affinity and specificity of proteins and peptides
Rapid screening of phage displayed protein binding affinities by phage ELISA
Identification of novel ligands for receptors using recombinant peptide libraries
Substrate phage display
Protease-based selection of stably folded proteins and protein domains from phage display libraries
Phage display of zinc fingers and other nucleic acid-binding motifs
In vivo and ex vivo selections using phage-displayed libraries
Screening phage libraries with sera
Interaction cloning using cDNA libraries displayed on phage
Phage antibody libraries
Affinity maturation of phage antibodies
