PCR Protocols
A Guide to Methods and Applications
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Product details:
- Publisher Elsevier Science
- Date of Publication 23 January 1990
- ISBN 9780123721808
- Binding Hardback
- No. of pages482 pages
- Size 234x158 mm
- Weight 910 g
- Language English 0
Categories
Long description:
The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual.
MoreTable of Contents:
[Contents in a condensed version]
Basic Methodology
Amplification of genomic DNA targets
Optimization of PCR reaction and design of primers
Nonradioactive detection of PCR products
mRNA quantitation by PCR
Cloning PCR products
Contamination and product carry-over
PCR from paraffin sections
Amplifying ancient DNA
Research Applications
Direct DNA sequencing of PCR products
DNA footprinting
Detection of rare mRNAs
Site-directed mutagenesis
Screening DNA libraries
Amplification of flanking DNA sequences
Genetics and Evoltion
Tissue typing and disease susceptibility
Haplotype analysis of single cells
Population biology
Multiplex DNA amplification
Diagnostics and Forensics
HIV detection
HTLV I and II detection
Screening for novel viruses
Detection of oncogenic mutations
Diagnosis of retinoblastoma
Individual identification
Instruments and Supplies
Organizing a laboratory for PCR work
Low-cost air-driven cycling oven
Basic equipment and supplies for the PCR lab
