Mucins
Series: Methods in Enzymology; 732;
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Product details:
- Publisher Elsevier Science
- Date of Publication 1 July 2026
- ISBN 9780443432262
- Binding Hardback
- No. of pages258 pages
- Size 229x152 mm
- Weight 450 g
- Language English 700
Categories
Long description:
Mucins, Volume 732 serves as a valuable resource for researchers aiming to investigate mucin biology in greater depth, offering a wide range of experimental approaches and analytical strategies. Topics in this new release cover Enrichment/isolation, glycoproteomics, Mucinase cleavage characterization and usage in glycoproteomics, Fluorescent protein-based FRET substrates for activity screening of mucinases, Development of clinical lectin assays based on MS glycomic discovery, Enzymatic selective sulfation of synthetic mucins, cryoEM, Cell-based mucin arrays and glycoengineering, CRISPR screens to characterize and identify glycan-binding proteins, and much more.
Additional sections cover MD Simulations, Interactions with pathogenic bacteria, Interactions with microbiota, Covalent crosslinking of mucins, Liquid Glycan and Liquid Lectin Array-based exploration of musin recognition, Quantification of mucins, Mucins in IBD, Investigations of mucins and innate immunity, Generating synthetic mucin glycopeptides, and Microscopy and imaging.
Table of Contents:
1. Enrichment/isolation, glycoproteomics
Stacy Malaker, Shane Finn and Sarah Lowery
2. Mucinase cleavage characterization and usage in glycoproteomics
Stacy Malaker, Alexandra Steigmeyer and Joann Chongsaritsinsuk
3. Fluorescent protein-based FRET substrates for activity screening of mucinases
Alisdair Boraston
4. Development of clinical lectin assays based on MS glycomic discovery
Niclas Karlsson, Ali Reza Afshari and Mary Solbego
5. Enzymatic selective sulfation of synthetic mucins
Jessica Kramer
6. cryoEM
Deborah Fass
7. Cell-based mucin arrays and glycoengineering
Yoshiki Narimatsu, Christian Bull, Henrik Clausen and Felix Goerdeler
8. CRISPR screens to characterize and identify glycan-binding proteins
Simon Wisnovsky
9. MD Simulations
Fiona Kearns
10. Interactions with pathogenic bacteria
Katharina Ribbeck
11. Interactions with microbiota
Eric Martens
12. Covalent crosslinking of mucins
Thomas Crouzier
13. Liquid Glycan and Liquid Lectin Array-based exploration of musin recognition
Ratmir Derda
14. Quantification of mucins
Sumaira Z. Hasnain
15. Mucins in IBD
Kirk Bergstrom
16. Investigatins mucins and innate immunity
Thaher Pelaseyed
17. Generating synthetic mucin glycopeptides
Peter Seeberger, Kabita Pradhan and Charlotte V-lkel
18. Microscopy and imaging
Malin E.V. Johansson, George M H Birchenough, Elisabeth E L Nystr-m and Brendan Dolan
